HPLC Separation of CBD, CBN, delta-9 THC, delta-8 THC on Cannsep C Column

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of CBD, CBN, delta-9 THC, delta-8 THC

Cannabidiol (CBD), cannabinol (CBN), and delta-9-Tetrahydrocannabinol (delta-9 THC) are three of the most active cannabinoids in cannabis. Delta-8-tetrahydrobannabinol also occurs naturally, but in such small amounts that it is often cheaper and easier to synthesize it in the laboratory from CBD. Both forms of THC are the strongest of the cannabinoids and are the main cause of the psychoactive effects of cannabis. CBD, while the 2nd most active component, does not cause the same effects by itself, but studies show it may have other neurologic and psychiatric benefits. There has been a lot less research conducted on CBN and its potential health benefits, but it is a product of the breakdown of THC and works on the same receptors (albeit with different effects) as CBD.
These cannabinoids can be detected in the low UV regime. Using a Cannsep C reverse-phase column and a mobile phase consisting of water and methanol (MeOH) with no buffer, CBD, CBN, delta-9 THC, and delta-8 THC can be separated, measured, and analyzed. This analysis method can be UV detected at 275 nm with high resolution.


Column Cannsep C, 2.1×100 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 82/18%
Buffer No
Flow Rate 0.5 ml/min
Detection UV, 275 nm



Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds  Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC),  Cannabinol (CBN), Delta-8-Tetrahydrocannabinol (THC),


Application Column

Cannsep C

The Cannsep family of columns is a specially developed line of reverse-phase columns designed to retain and separate most of the compounds responsible for cannabis's physiological properties. With three options available - Cannsep A, Cannsep B, and Cannsep C - customers can resolve all the various cannabinoids, each providing unique and significantly orthogonal selectivity.

Select options
Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.