HPLC Determination of CBD, CBN, Δ9-THC, Δ8-THC on Cannsep C Column

HPLC Method for Cannabidiol (CBD), Cannabinol (CBN), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC) on Cannsep C by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cannabidiol (CBD), Cannabinol (CBN), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC).

Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. THC has psychoactive effects, while CBD does not.

They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.

Condition

Column Cannsep C, 3.2 x 150 mm, 3 µm, 100 A, dual ended
Mobile Phase MeOH -80%,
Buffer H2SO4 – 0.2%
Flow Rate 0.5 ml/min
Detection UV, 200 nm

 

Description

Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds Cannabidiol (CBD), Cannabinol (CBN), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC)

 

Application Column

Cannsep C

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.