HPLC Determination of CBD, CBN, Δ9-THC, Δ8-THC on Cannsep C Column

HPLC.cloud View on hplc.cloud Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.


Column Cannsep C, 3.2x150 mm, 3 µm, 100A
Mobile Phase MeOH -80%,
Buffer H2SO4 - 0.2%
Flow Rate 0.5 ml/min
Detection UV, 200 nm


Class of Compounds Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds  Cannabidiol (CBD) Delta-9-Tetrahydrocannabinol (THC), CBD, CBN, Δ9-THC, Δ8-THC
Application Analytes:
Cannabidiol (CBD)
Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)