HPLC Method for Cannabidiol (CBD), Cannabinol (CBN), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC) on Cannsep C by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Cannabidiol (CBD), Cannabinol (CBN), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC).
Δ8-THC, Δ9-THC, CBD and CBN are cannabinoids found in Cannabis plants. They have some medical uses among which is the prevention of nausea and vomiting caused by some cancer medications. THC has psychoactive effects, while CBD does not.
They can be separated using HPLC on a reverse-phase Cannsep C column using a mobile phase consisting of methanol (MeOH) and water with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.
| Column | Cannsep C, 3.2 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeOH -80%, |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Drug, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | Cannabidiol (CBD), Cannabinol (CBN), Delta-8-Tetrahydrocannabinol (8-THC), Delta-9-Tetrahydrocannabinol (THC) |
Application Column
Cannsep C
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Cannabidiol (CBD)Cannabinol (CBN)
Delta-8-Tetrahydrocannabinol (8-THC)
Delta-9-Tetrahydrocannabinol (THC)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
