HPLC Separation of Organic Acids in HILIC Mode on Primesep N Column

Ascorbic, methylmalonic and succinic are weak organic acids. Retention of these three acids is achieved on Primesep N column in HILIC mode using acetonitrile/water and ammonium acetate. Compounds are monitored by ELSD. Method can be used for determination of ascorbic acid (Vitamin C), methylmalonic acid and succinic acid in various matrices. Other polar organic acids can be analyzed on this HILIC column.


Column Primesep  N , 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Acid, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Ascorbic acid (Vitamin C), Methylmalonic acid,  Succinic acid

Application Analytes:
Ascorbic Acid
Methylmalonic Acid
Organic Acids
Succinic Acid