| CAS Number | 516-05-2 |
|---|---|
| Molecular Formula | C4H6O4 |
| Molecular Weight | 118.088 |
| InChI Key | ZIYVHBGGAOATLY-UHFFFAOYSA-N |
| LogP | -0.367 |
| Synonyms |
|
Applications:
HPLC Method for Analysis of Dibasic Acids on Newcrom B Column
July 29, 2022
Separation type: Liquid Chromatography Mixed-mode
Succinic acid is a key intermediary (in the form of its anion, succinate) in the electron transport chain, a process that is the key power source for our cells. It also has a few limited functions outside of the mitochondria. Glutaric acid is a common plasticizer precursor and often used in the production of polyester polyols and polyamides. Interestingly, it is significantly more water soluble than either Succinic or Adipic acid. Adipic acid, also known as hexanedioic acid, is a key precursor to nylon, and more than 2.5 billion kilograms are produced every year. Methylmalonic acid is a key intermediary in the anaplerotic reactions of the Krebs cycle. These Dibasic acids can be separated, retained, and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (FA). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Adipic Acid, Succinic Acid, Glutaric Acid, Methylmalonic acid
| Column | Newcrom B, 4.6×150 mm, 100A |
| Mobile Phase | MeCN/H2O -5/95% |
| Buffer | H2SO4 – 0.02% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Adipic Acid, Succinic Acid, Glutaric Acid, Methylmalonic acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsGlutaric acid
Methylmalonic Acid
Succinic Acid
HPLC Separation of Methylmalonic and Malonic Acid on Newcrom B Column
November 20, 2019
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H3PO4 – 0.02% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid, Hydrophilic, Polar |
| Analyzing Compounds | Methylmalonic acid, Malonic Acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsMethylmalonic Acid
HPLC Separation of Methylmalonic, Fumaric, Maleic acids on Newcrom B Column
October 28, 2019
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeOH/H2O – 5/93% |
| Buffer | Formic Acid – 2% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Methylmalonic acid, Fumaric acid, Maleic acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsMaleic Acid
Methylmalonic Acid
HPLC Separation of Small Organic Acids on Newcrom B Column
October 22, 2019
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | AmAc pH 5.0, Formic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds | Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Glycolic acid, Succinic Acid, Methylmalonic acid, Malic Acid, Maleic Acid, Citric Acid, Tartaric Acid, Fumaric Acid, Malonic Acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsFumaric Acid
Gluconic acid
Glycolic acid
Maleic Acid
Malic Acid
Malonic Acid
Methylmalonic Acid
Succinic Acid
Tartaric Acid
dl-Tartaric acid
HPLC Separation of Succinic and Methylmalonic Acids
November 12, 2010
Organic and inorganic acids can be separated by reversed-phase anion-exchange Primesep D column. Succinic and methylmalonic acids were separated based on their hydrophobic and ionic properties. Method can be used for analysis of other acids by HPLC.
Application Column
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsOrganic Acids
Succinic Acid
HILIC Separation of Carboxylic Acids
August 22, 2008
Hydrophilic acids are separated on Obelisc N mixed-mode HILIC column. Seven carboxylic acids are separated based on their polarity and pKa values. Changes in ionization states of acids and stationary phase can be used to control elution order of organic and inorganic acids.
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsHydroxybenzoic Acid
Malic Acid
Mandelic Acid
Methylmalonic Acid
Organic Acids
Succinic Acid
Tartaric Acid
HPLC Separation of Organic Acids in HILIC Mode on Primesep N Column
December 6, 2007
Ascorbic, methylmalonic and succinic are weak organic acids. Retention of these three acids is achieved on Primesep N column in HILIC mode using acetonitrile/water and ammonium acetate. Compounds are monitored by ELSD. Method can be used for determination of ascorbic acid (Vitamin C), methylmalonic acid and succinic acid in various matrices. Other polar organic acids can be analyzed on this HILIC column.
| Column | Primesep N , 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Acid, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Ascorbic acid (Vitamin C), Methylmalonic acid, Succinic acid |
Application Column
Primesep N
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsMethylmalonic Acid
Organic Acids
Succinic Acid
