HPLC Separation of Methylmalonic and Malonic Acid on Newcrom B Column

HPLC Method for Methylmalonic Acid, Malonic Acid on Newcrom B by SIELC Technologies

HPLC Separation of Methylmalonic and Malonic Acid on Newcrom B Column_1199

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Methylmalonic Acid, Malonic Acid .

Methylmalonic Acid is an organic acid with the chemical formula  C4H6O4. It works as an intermediate in the breakdown of certain amino acids and fatty acids. Elevated levels of Methylmalonic avid can be a sign of a Vitamin B12 deficiency or Methylmalonic acidemia, which is a rare genetic disorder where the previously mentioned acid is not properly metabolized, leading to its accumulation.

Malonic Acid is an organic acid with the C3H4O4 chemical formula.  It has a variety of uses from synthesis to preservatives. When it comes to synthesis, it is often used in industrial means. and especially dyes for natural fibers. Besides industrial use, in laboratory environments, it is used in preparation of tris-maleate, sodium maleate buffers, and maleate salts.

Methylmalonic Acid, Malonic Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a triethanolamine buffer. Detection is performed using UV.

Condition

Column Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.02%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid, Hydrophilic, Polar
Analyzing Compounds Methylmalonic Acid, Malonic Acid

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Malonic Acid
Methylmalonic Acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.