Separation type: Liquid Chromatography Mixed-mode
Succinic acid is a key intermediary (in the form of its anion, succinate) in the electron transport chain, a process that is the key power source for our cells. It also has a few limited functions outside of the mitochondria. Glutaric acid is a common plasticizer precursor and often used in the production of polyester polyols and polyamides. Interestingly, it is significantly more water soluble than either Succinic or Adipic acid. Adipic acid, also known as hexanedioic acid, is a key precursor to nylon, and more than 2.5 billion kilograms are produced every year. Methylmalonic acid is a key intermediary in the anaplerotic reactions of the Krebs cycle. These Dibasic acids can be separated, retained, and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of water, Acetonitrile (MeCN), and Sulfuric acid (FA). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Adipic Acid, Succinic Acid, Glutaric Acid, Methylmalonic acid
| Column | Newcrom B, 4.6×150 mm, 100A |
| Mobile Phase | MeCN/H2O -5/95% |
| Buffer | H2SO4 – 0.02% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Adipic Acid, Succinic Acid, Glutaric Acid, Methylmalonic acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsGlutaric acid
Methylmalonic Acid
Succinic Acid
