HPLC Separation of Methylmalonic, Fumaric, Maleic acids on Newcrom B Column


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Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 5/93%
Buffer Formic Acid – 2%
Flow Rate 1.0 ml/min
Detection CAD (Corona)  (MS-compatible mobile phase)

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Methylmalonic acid, Fumaric acid, Maleic acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.

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Application Analytes:
Fumaric Acid
Maleic Acid
Methylmalonic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.