HPLC Separation of Small Organic Acids on Newcrom B Column


HPLC.cloud
View on hplc.cloud

 

Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 5/95%
Buffer AmAc pH 5.0, Formic Acid
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description

Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Glycolic acid, Succinic Acid, Methylmalonic acid, Malic Acid, Maleic Acid, Citric Acid, Tartaric Acid, Fumaric Acid, Malonic Acid

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Citric Acid
Fumaric Acid
Gluconic acid
Glycolic acid
Maleic Acid
Malic Acid
Malonic Acid
Methylmalonic Acid
Succinic Acid
Tartaric Acid
dl-Tartaric acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.