| CAS Number | 16887-00-6 |
|---|---|
| Molecular Formula | Cl |
| Molecular Weight | 35.450 |
| InChI Key | VEXZGXHMUGYJMC-UHFFFAOYSA-M |
| LogP | 0.0 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Inorganic anions on BIST™A+ Column
2022-07-07
| Separation type: Bridge Ion Separation Technology, or BIST™ | ||||||||||||||
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| High Performance Liquid Chromatography (HPLC) Method for Analysis of Inorganic anions, Chloride, Bromide, Nitrate, Iodide, Perchlorate
Using SIELC’s newly introduced BIST™ method, a mixture of many different inorganic anions can be separated on a negatively-charged, cation-exchange BIST™ A column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry. |
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Application Analytes:
BromideChloride
Iodide
Methanesulfonic Acid
Nitrate
Perchlorate
TFA (Trifluoroacetic Acid)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
BIST A
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select options
BIST A+
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select optionsHPLC Determination of Chloride, Chlorate and Perchlorate on Newcrom B Column
2019-12-10
Main source of contamination of environment by perchlorates are rocket fuels, car airbags, and fireworks.
EPA developed regulation of the perchlorate level in drinking water which already adapted by several states.
The most convenient, universal and very sensitive way to measure perchlorate is chromatography.
SIELC developed a simple, rugged, and selective HPLC method which allows to measure perchlorate in different matrices including drinking water.
This method allows to measure simultaneously other chloro containing ions such as chloride, and chlorate.
The method shows high selectivity and specificity.
The mobile phase is a simple mixture of water acetonitrile and ammonium formate.
The column used in the analysis has advanced surface chemistry with long chain holding a terminal positively changed functional group.
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | Gradient AmFm pH 3.0 – 40-100 mM , 10 min |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium Chloride, Sodium Chlorate, Sodium Perchlorate |
Application Analytes:
ChlorateChloride
Perchlorate
Sodium Chlorate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Newcrom B
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 columns are standard reverse phase columns with octyldecyl silane chains (C18) attached to porous silica. We have also designed a line of Newcrom R1 columns with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).
Select optionsHPLC Separation of Inorganic Anions on Newcrom BH Column
2019-10-23
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | Gradient AmAc pH 5.0 – 20-90 mM , 20 min |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Phosphate, Chloride, Bromide, Nitrate, Iodide, Sulfate, Perchlorate |
Application Analytes:
BromideChloride
Iodide
Iodine
Nitrate
Perchlorate
Phosphate
Sodium
Sulfate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Newcrom BH
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 columns are standard reverse phase columns with octyldecyl silane chains (C18) attached to porous silica. We have also designed a line of Newcrom R1 columns with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).
Select optionsSeparation of Paraquat, Diguat, and Glyphosate on Obelisc R Column
2013-07-03
Paraquat, diquat and glyphosate are three of most widely used herbicides in the world. Paraquat and diquat are very polar and very basic quaternary amines. Glyphosate is an aminophosphonic analog of glycine. It is very polar and acidic at most of the pH of the mobile phase. Since glyphosate and the quats have opposite charges no ion-pairing method can be developed for the mixture of basic and acidic herbicides. All three herbicides were separated on the Obelisc R tri-modal column. Paraquat and diquat are retained by a cation-exchange mechanism, and glyphosate is retained by weak reversed-phase and strong anion-exchange mechanisms. This method can be used for analysis of common herbicides in fruits, vegetables, ground water, drinking water and other matrices. Method is LC/MS compatible and can be used to determine trace levels of herbicides.
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 30% |
| Buffer | Gradient AmFm pH 3.0 – 5-30 mM, 10 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 250 nm, ELSD |
| Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Paraquat, Diquat, Glyphosate |
Application Analytes:
ChlorideDiquat
Glyphosate
Paraquat
Sodium
Application Detection:
UV DetectionELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Obelisc R
SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - Buffer concentration, Buffer pH, and Organic Modifier Concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsHPLC Separation of Mepiquat on Obelisc R Column
2011-07-08
Mepiquat is a plant growth regulator that is monitored by EPA in water. It is a quaternary amine that is very polar in nature. Mepiquat and other hydrophilic amines are not retained on reversed-phase columns, and produce poor peak shape due to residual silanol interactions. Ion-pairing reagent is required to retain mepiquat. Ion-pairing reagents are not compatible with LC/MS detection and most environmental application require high sensitivity methods to determine very low level of contaminants. Obelisc R mixed-mode column can be used for EPA methods for determination of common pesticides, herbicides and insecticides where analysis of very polar ionic compounds is required.
| Column | Obelisc R, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds |
Grow regulator, Hydrophilic, Ionizable |
| Analyzing Compounds | Mepiquat, Chloride |
Application Analytes:
ChlorideMepiquat
Application Detection:
ELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Obelisc R
SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - Buffer concentration, Buffer pH, and Organic Modifier Concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsHPLC Separation of Sodium, Chloride, Bromide, and Iodine
2010-11-21
Halide ions are usually analyzed by ion-chromatography with a conductivity detector. Mixed-mode chromatography in combination with ELSD is offering a valuable alternative to ion-chromatography with conductivity detection. Chloride, bromide and iodide were separated on a Primesep SB mixed-mode anion-exchange column. Mobile phase is compatible with ELSD and LC/MS.
| Column | Primesep SB, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Hydrophilic, Ions |
| Analyzing Compounds | Chloride, Sodium, Bromide, Iodine |
Application Analytes:
BromideChloride
Iodine
Sodium
Application Detection:
ELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Primesep SB
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsHPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Obelisc N
2010-03-03
Ion chromatography is usually used for analysis of hydrophilic organic and inorganic ions. Same separation can be achieved on HILIC/mixed-mode Obelisc N HPLC columns. Obelisc N HPLC columns have very polar groups on their surface: one of the groups is basic and the other acidic. In case of low organic concentration, two groups are connected by hydrophilic linker. Obelisc N column can be used as cation-exchange and anion-exchange column. This allows to separate positively and negatively charged molecules in one run. Five anions (chloride, bromide, methanesulfonate, nitrate and perchlorate) along with one cation (sodium) were separated in one run. Method is compatible with ELSD, CAD and LC/MS and can be used for analysis of various hydrophilic and hydrophobic cations and anions in one HPLC run.
| Column | Obelisc N, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Hydrophilic, Ions |
| Analyzing Compounds | Chloride, Nitrate, Chlorate, Bromide, Potassium |
Application Analytes:
BromideChloride
Methanesulfonic Acid
Nitrate
Nitric Acid
Potassium
Application Detection:
ELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Obelisc N
SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - Buffer concentration, Buffer pH, and Organic Modifier Concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsHPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography
2009-07-16
The majority of drugs in the pharmaceutical industry are administered in salt form. The presence of two counter-ions very often necessitates the use of two methods. The nature of these counterparts in drugs can be an inorganic cation and organic acid, inorganic anion and organic base, and organic cation and organic anion. Furthermore, the properties of the molecules will result in a differing stoichiometry. The task of simultaneous quantitation of counter-ions can be achieved by using mixed-mode columns. The general approach for analysis is based on properties of corresponding counter-ions. Hydrophobic basic drugs, like dextromethorphan, verapamil, trimipramine, and corresponding acidic counter-ions (chloride, chlorate, bromide, bromate, perchlorate, maleate, fumarate,tartrate, succinate, phosphate, citrate, benzosulfonate, toleuensulfonate) can be separated and quantitated in the same run on reversed-phase anion-exchange column. Basic hydrophobic drugs are retained by the reversed-phase mechanism, and counter-ions are retained by the reversed-phase and anion-exchange mechanism. Some polar counter-ions are retained only by the anion-exchange mechanism. Retention time and selectivity of HPLC separation of drugs and counter-ions can be achieved by changing the amount of acetonitrile and the amount of ions in the mobile phase. The detection technique depends on the properties of the counter-ions. In case of low or no UV activity, ELSD can be employed if the counter-ion forms a non-volatile salt with the mobile phase additive (ammonium formate). This HPLC method can be used for simultaneous quantitation of other basic drugs and counter-ions. The presence of two mechanisms of retention allows control over retention times of drug and counter-ion independently, and even allows a change of order of elution when necessary.
| Column | Primesep D , 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, UV 270 |
| Class of Compounds | Ions, Hydrophilic, Hydrophobic, Base, Acids, Ionizable |
| Analyzing Compounds | Sodium Chloride, Sodium chloride, Sodium Chlorate, Sodium bromide, Sodium bromate, Perchloric Acid, Maleic Acid, Fumaric Acid, Tartaric Acid, Succinic Acid, Phosphoric Acid, Citric acid, Benzosulfonic acid, Dextromethorphan, Verapamil, Trimipramine |
Application Analytes:
Benzenesulfonic AcidBromide
Chlorate
Chloride
Citric Acid
Dextromethorphan
Fumaric Acid
Maleic Acid
Organic Acids
Perchlorate
Phosphoric Acid
Pyrilamine
Succinic Acid
Tartaric Acid
Verapamil
p-Toluenesulfonic Acid (PTSA)
Application Detection:
UV DetectionELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Primesep D
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsEffect of Concentration of Organic Modifier on Retention of Cation and Anions
2005-11-20
Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium cations by cation exchange. The retention can be adjusted by changing the water/acetonitrile ratio in the mobile phase. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate with evaporative light scattering detection (ELSD).
| Column | Primesep 100, Primesep B , 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 5.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Potassium, Bromide, Chloride |
Application Analytes:
BromideChloride
Potassium
Sodium
Application Detection:
ELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Primesep 100
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select options
Primesep B
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsSimultaneous Separation of Cations and Anions
2005-11-15
Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium captions by cation exchange. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD).
| Column | Primesep 100, Primesep B , 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 5.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds |
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Potassium, Bromide, Chloride |
Application Analytes:
BromideChloride
Potassium
Sodium
Application Detection:
ELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Primesep 100
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select options
Primesep B
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsSeparation of Zn and Mn on Primesep 100 Column
2005-10-15
Primesep 100 separates the divalent cations, zinc and manganese, from their counter ion, chloride. The cations are retained by cation exchange and the chloride anion is eluted by ion exclusion. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate is evaporative light scattering detection (ELSD) compatible.
| Column | Primesep 100, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 4.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Chloride, Magnesium, Zinc |
Application Analytes:
ChlorideManganese
Zinc
Application Detection:
ELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Primesep 100
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsZn and Na Quantitation in Drug Substances
2005-10-11
Primesep 100 allows the quantitation of sodium and zinc cations in a drug product sample. The cations are retained by cation exchange and the drug product is retained by hydrophobic, reversed-phase mechanisms. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD) and UV detection.
| Column | Primesep 100, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds |
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Zinc |
Application Analytes:
ChlorideSodium
Zinc
Application Detection:
UV DetectionELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Primesep 100
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsSeparation of Metal Ions on Primesep 100 Column
2005-10-04
Primesep 100 separates the monovalent cations, lithium, potassium, and sodium, and the divalent cations, zinc, manganese, and calcium. The cations are resolved by cation exchange. The mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) is evaporative light scattering detection (ELSD) compatible.
| Column | Primesep 100, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD 50C |
| Class of Compounds |
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Phosphate, Magnesium, Calcium, Lithium, Zinc |
Application Analytes:
CalciumChloride
Lithium
Magnesium
Potassium
Sodium
Zinc
Application Detection:
ELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Primesep 100
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsHPLC Separation of Chloride and Nitrate Ions
2005-01-13
High nitrate concentrations in water and soil can be toxic to humans, fish and domestic animals. Chloride can be used for analysis of water contamination. Primesep D column can be used to separate and quantify both ions. The baseline separation between chloride and nitrate can be increased by decreasing the ammonium formate concentration in the mobile phase. Evaporative Light Scattering Detection (ELSD) used.
| Column | Primesep D, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Hydrophilic, Ion |
| Analyzing Compounds | Chloride, Nitrate |
Application Analytes:
ChlorideNitrate
Nitric Acid
Sodium
Application Detection:
ELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Primesep D
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select options