HPLC Method for Analysis of Inorganic anions on BIST™A+ Column

 

Separation type: Bridge Ion Separation Technology, or BIST™
 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Inorganic anions, Chloride, Bromide, Nitrate, Iodide,  Perchlorate

Using SIELC’s newly introduced BIST™ method, a mixture of many different inorganic anions can be separated on a negatively-charged, cation-exchange BIST™ A column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry.

Condition
Column BIST™ A+, 4.6×50 mm, 5µm, 100A
Mobile Phase MeCN – 90%
Buffer TMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) formate – 5 mM pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD, 70C

 

Description
Class of Compounds
  Acid, Inorganic anion
Analyzing Compounds Chloride, Bromide, Nitrate, Iodide,  Perchlorate, TFA, Methanesulfonic acid

Application Column

BIST A

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Bromide
Chloride
Iodide
Methanesulfonic Acid
Nitrate
Perchlorate
TFA (Trifluoroacetic Acid)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.