HPLC Method for Analysis of Inorganic anions on BIST™A+ Column

Using SIELC’s newly introduced BIST™ method, a mixture of many different inorganic anions can be separated on a negatively-charged, cation-exchange BIST™ A column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry.