Perchlorate

CAS Number14797-73-0
Molecular FormulaClO4
Molecular Weight99.450
InChI KeyVLTRZXGMWDSKGL-UHFFFAOYSA-M
LogP-4.63
Synonyms
  • Perchlorate
  • Perchlorato, ion(1-)
  • 14797-73-0
  • Perchlorate ion
  • Perchlorate ion (ClO41-)
  • Perchlorate ion(1-)
  • Perchlorate(1-)
  • Perchloric acid, ion(1-)
  • Perchlorate ions
  • UNII-VLA4NZX2P4
  • 181259-57-4
  • 60349-26-0

Applications:

HPLC Method for Analysis of Inorganic anions on BIST™A+ Column

2022-07-07

 

Separation type: Bridge Ion Separation Technology, or BIST™
 
High Performance Liquid Chromatography (HPLC) Method for Analysis of Inorganic anions, Chloride, Bromide, Nitrate, Iodide,  Perchlorate Using SIELC’s newly introduced BIST™ method, a mixture of many different inorganic anions can be separated on a negatively-charged, cation-exchange BIST™ A column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N',N'-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry.

Condition

Column BIST™ A+, 4.6x50 mm, 5µm, 100A
Mobile Phase MeCN - 90%
Buffer TMDAP ( N,N,N',N'-Tetramethyl-1,3-diaminopropane) formate – 5 mM pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD, 70C
 

Description

Class of Compounds  Acid, Inorganic anion
Analyzing Compounds Chloride, Bromide, Nitrate, Iodide,  Perchlorate, TFA, Methanesulfonic acid

Application Analytes:
Bromide
Chloride
Iodide
Methanesulfonic Acid
Nitrate
Perchlorate
TFA (Trifluoroacetic Acid)

HPLC Separation of Polar Pesticides on Newcrom B Column

2020-11-19
HPLC.cloud View on hplc.cloud               Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. All are heavily used in agriculture. By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom B mixed-mode column.

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer AmFm pH 3.0 Gradient  5 - 60 mM 15 min
Flow Rate 1 ml/min
Detection CAD
 

Condition 2

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer AmFm pH 3.0 Gradient  5 - 15 mM 10 min
Flow Rate 1 ml/min
Detection CAD
   

Description

Class of Compounds Pesticides, Herbicides, Fungicides, Insecticides
Analyzing Compounds Aminomethylphosphonic acid (AMPA), Bialaphos, Glyphosate, Phosphorous acid H3PO3, Fosetyl-Al, Bromide, Chlorate, Perchlorate, 3-Methylphosphinicopropionic acid (MPPA), Bromide
 
Application Analytes:
3-(Methylphosphinico)propionic acid
Aminomethylphosphonic acid (AMPA)
Bialaphos
Bromate
Bromide
Chlorate
Fosetyl-Al
Glyphosate
Perchlorate
Phosphorous acid
Sodium Bromate

HPLC Determination of Chloride, Chlorate and Perchlorate on Newcrom B Column

2019-12-10
HPLC.cloud View on hplc.cloud Main source of contamination of environment by perchlorates are rocket fuels, car airbags, and fireworks. EPA developed regulation of the perchlorate level in drinking water which already adapted by several states. The most convenient, universal and very sensitive way to measure perchlorate is chromatography. SIELC developed a simple, rugged, and selective HPLC method which allows to measure perchlorate in different matrices including drinking water. This method allows to measure simultaneously other chloro containing ions such as chloride, and chlorate. The method shows high selectivity and specificity. The mobile phase is a simple mixture of water acetonitrile and ammonium formate. The column used in the analysis has advanced surface chemistry with long chain holding a terminal positively changed functional group.

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer Gradient AmFm pH 3.0 -  40-100 mM , 10 min
Flow Rate 1.0 ml/min
Detection CAD

Description

Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Sodium Chloride, Sodium Chlorate, Sodium Perchlorate

Application Analytes:
Chlorate
Chloride
Perchlorate
Sodium Chlorate

HPLC Separation of Inorganic Anions on Newcrom BH Column

2019-10-23
HPLC.cloud View on hplc.cloud      

Condition

Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer Gradient AmAc pH 5.0 -  20-90 mM , 20 min
Flow Rate 1.0 ml/min
Detection CAD (Corona)  (MS-compatible mobile phase)

Description

Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Phosphate, Chloride, Bromide, Nitrate, Iodide, Sulfate,  Perchlorate

Application Analytes:
Bromide
Chloride
Iodide
Iodine
Nitrate
Perchlorate
Phosphate
Sodium
Sulfate

Separation of Chlorate, Perchlorate, and Phosphonate Ions

2015-07-30
  The ionic forms of Chlorate, Perchlorate, and Phosphonates are useful in many industries including medicine, paper and use in explosives. Due to their lack of UV activity, an ELSD was used to detect both the anions and cations of all three sodium salts. The ions were retained on both Primesep D and Obelisc R columns. Primesep D is a reverse phase column with embedded basic ion-pairing groups. Obelisc R is also a reverse phase column, but can be additionally tuned due to embedded ionic groups and a hydrophobic chain.

Condition 1

Column Primesep D, 2.1x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-40%, 12 min
Buffer Gradient AmFm pH 2.3- 30-80 mM, 12 min
Flow Rate 0.4 ml/min
Detection ELSD
 

Condition 2

Column Obelisc R, 2.1x150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 10-40%, 12 min
Buffer Gradient AmFm pH 2.3- 30-80 mM, 12 min
Flow Rate 0.4 ml/min
Detection ELSD
   

Description

Class of Compounds   Hydrophilic, Ionizable
Analyzing Compounds Chlorate, Perchlorate,  Phosphonate Ions
 
Application Analytes:
Chlorate
Perchlorate
Phosphonate
Sodium

Application Detection:
ELSD/MS Detection

HPLC Analysis of Basic Drugs and Acidic Counter-Ions by Mixed-Mode Chromatography

2009-07-16
The majority of drugs in the pharmaceutical industry are administered in salt form. The presence of two counter-ions very often necessitates the use of two methods. The nature of these counterparts in drugs can be an inorganic cation and organic acid, inorganic anion and organic base, and organic cation and organic anion. Furthermore, the properties of the molecules will result in a differing stoichiometry. The task of simultaneous quantitation of counter-ions can be achieved by using mixed-mode columns. The general approach for analysis is based on properties of corresponding counter-ions. Hydrophobic basic drugs, like dextromethorphan, verapamil, trimipramine, and corresponding acidic counter-ions (chloride, chlorate, bromide, bromate, perchlorate, maleate, fumarate,tartrate, succinate, phosphate, citrate, benzosulfonate, toleuensulfonate) can be separated and quantitated in the same run on reversed-phase anion-exchange column. Basic hydrophobic drugs are retained by the reversed-phase mechanism, and counter-ions are retained by the reversed-phase and anion-exchange mechanism. Some polar counter-ions are retained only by the anion-exchange mechanism. Retention time and selectivity of HPLC separation of drugs and counter-ions can be achieved by changing the amount of acetonitrile and the amount of ions in the mobile phase. The detection technique depends on the properties of the counter-ions. In case of low or no UV activity, ELSD can be employed if the counter-ion forms a non-volatile salt with the mobile phase additive (ammonium formate). This HPLC method can be used for simultaneous quantitation of other basic drugs and counter-ions. The presence of two mechanisms of retention allows control over retention times of drug and counter-ion independently, and even allows a change of order of elution when necessary.

Condition

Column Primesep D , 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD, UV 270

Description

Class of Compounds Ions, Hydrophilic, Hydrophobic, Base, Acids, Ionizable
Analyzing Compounds Sodium Chloride, Sodium chloride, Sodium Chlorate, Sodium bromide, Sodium bromate, Perchloric Acid, Maleic Acid, Fumaric Acid, Tartaric Acid, Succinic Acid, Phosphoric Acid, Citric acid, Benzosulfonic acid,  Dextromethorphan, Verapamil, Trimipramine

Application Analytes:
Benzenesulfonic Acid
Bromide
Chlorate
Chloride
Citric Acid
Dextromethorphan
Fumaric Acid
Maleic Acid
Organic Acids
Perchlorate
Phosphoric Acid
Pyrilamine
Succinic Acid
Tartaric Acid
Verapamil
p-Toluenesulfonic Acid (PTSA)

Application Detection:
UV Detection
ELSD/MS Detection