HPLC Method for Separation and Analysis of N-Acetyl-S-(3-hydroxypropyl)-L-cysteine, 2-Hydroxyisobutyric acid, Perchlorate, N-Acetyl (Carbamoylmethyl) cysteine, Diphenyl phosphate, N-Acetyl-S-propyl-L-cysteine with an MS-Compatible Mobile Phase on a Newcrom B Column by SIELC Technologies.

Metabolites and biomarkers are key terms in the fields of biochemistry, pharmacology, and medicine.

N-Acetyl-S-(3-hydroxypropyl)-L-cysteine (3HPMA) is a common metabolite of the herbicide and pollutant Acrolein with the chemical formula C₈H₁₅NO₄S. It is primarily used to check for tobacco and pollutant exposure in urine samples.

2-Hydroxyisobutyric Acid (2HIB) is a neuro and adrenal toxin with the chemical formula C₄H₈O₃. It is a common metabolite of the fuel additives Methyl tert-butyl ether (MTBE) and Ethyl tert-butyl ether (ETBE).

N-Acetyl(Carbomylethyl)cysteine (NAE) is a metabolite of Acrylamide which can act as a neuro toxin in cases of long term exposure. It has the chemical formula C₈H₁₄N₂O₄S.

N-Acetyl(propyl)cysteine (NAPR) is a metabolite of the popular industrial organic solvent 1-bromopropane (1-BP) with the chemical formula C₈H₁₅NO₃S. It is s biomarker that forms in the urinary tract when the body detoxifies industrial propyl-based solvents.

Perchlorate, also written as PERC, is an inorganic compound with the formula ClO₄^–. It is most typically used for explosives. For example, fireworks and rocket propellants. Due to it’s water solubility, it can easily contaminate it. When ingested, it can pose a health risk and cause developmental harm. It inhibits thyroid activity and can be used to treat hyperthyroidism, but can be toxic in large amounts or in regularly-functioning thyroids.

Diphenylphosphate (DPP) is a metabolite of the popular flame retardant triphenyl phosphate (TPHP) with the chemical formula C₁₂H₁₁O₄P. It is produced when the body breaks down certain organophosphate glame retardants and is used in medical setting to track contact with the items.

N-Acetyl-S-(3-hydroxypropyl)-L-cysteine, 2-Hydroxyisobutyric acid, Perchlorate, N-Acetyl (Carbamoylmethyl) cysteine, Diphenyl phosphate, N-Acetyl-S-propyl-L-cysteine can be retained, separated, and analyzed on a mixed-mode [columnn] column with a mobile phase consisting of water, Acetonitrile (MeCN), and Ammonium Acetate (AmAc). This analytical method can be detected with high resolution and peak symmetry with many evaporative detection methods, including Evaporative Light Scattering Detection (ELSD), Charged Aerosol Detector (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).

Condition

Description