| CAS Number | 14265-44-2 |
|---|---|
| Molecular Formula | O4P |
| Molecular Weight | 94.971 |
| InChI Key | NBIIXXVUZAFLBC-UHFFFAOYSA-K |
| LogP | -0.770 |
| Synonyms |
|
Applications:
HPLC Determination of Fructose 1,6 Bisphosphate on Newcrom B Column
May 13, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Fructose 1,6 Bisphosphate
Fructose 1,6-biphosphate, alternatively known as Harden-Young ester, is the main form glucose and fructose take when they enter cells. It is a key component of the glycolysis metabolic pathway and is the product of the phosphorylation of fructose 6-phosphate.
Using a Newcrom B mixed-mode column and a mobile phase consisting of water and acetonitrile with an ammonium formate (AmFm) buffer, Fructose 1,6-bisphosphate can be retained, separated (from fructose 6-phosphate), and measured. Fructose 1,6 bisphosphate can be detected using charged aerosol detection (CAD) with high resolution.
| Column | Newcrom B, 4.6×150 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | AmFm – pH 3.0 – 20 mm |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds |
Sugar Phosphate |
| Analyzing Compounds | Sodium, Fructose 6 – phosphate, Fructose 1.6 biphosphate |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsFructose 6-Phosphate
Phosphate
HPLC Separation of Glyphosate, Glyphosine, Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column
December 4, 2019
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN Gradient -40-10%, 15 min |
| Buffer | Formic Acid Gradient -1- 5%, 15 min |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
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| Class of Compounds | Acids, Plant growth regulator, Herbicide, Hydrophilic, Ionizable |
| Analyzing Compounds | Ethylphosphonic acid, Phosphate Ion, Methylphosphonate, Methylenediphosphonic acid, Glyphosate, Glyphosine |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsGlyphosate
Glyphosine
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate
Phosphoric Acid
HPLC Separation of Glyphosate, Ethylphosphonic Acid and Methylphosphonic Acid on Newcrom B Column
December 2, 2019
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 40/59% |
| Buffer | Formic Acid – 1% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Ethylphosphonic acid, Phosphoric Acid, Methylphosphoric Acid, Glyphosate |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsGlyphosate
Methylphosphonic Acid
Phosphate
HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column
December 2, 2019
Separation type: Liquid Chromatography Mixed-mode
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosate and Phosphate Ion
Glyphosate is a broad-spectrum herbicide which is used to kill weeds. The presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Glyphosate is characterized by its carboxylic acid and phosphate group on either end.
Glyphosate and phosphate ions can be retained and separated with a Newcrom B mixed-mode column using a mobile phase consisting of acetonitrile (MeCN), water, and formic acid (H3PO4) as a buffer. This method is compatible with mass spectrometry and be detected via a Charged aerosol detector (CAD).
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 20% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Phosphate, Glyphosate |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsPhosphate
HPLC Separation of Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column
December 2, 2019
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 20/78% |
| Buffer | Formic Acid – 2% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Acids, Hydrophilic, Ionizable |
| Analyzing Compounds | Ethylphosphonic acid, Phosphoric, Methylphosphonic Acid, Methylenediphosphonic acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsMethylenediphosphonic acid
Methylphosphonic Acid
Phosphate
Phosphoric Acid
HPLC Separation of Inorganic Anions on Newcrom BH Column
October 23, 2019
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | Gradient AmAc pH 5.0 – 20-90 mM , 20 min |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Phosphate, Chloride, Bromide, Nitrate, Iodide, Sulfate, Perchlorate |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsChloride
Iodide
Iodine
Nitrate
Perchlorate
Phosphate
Sodium
Sulfate
HPLC Analysis of Sulfate and Phosphate Ions on Primesep Columns
October 14, 2010
Inorganic and organic ions are usually analyzed by ion-exchange mechanism. In this application, we used short Primesep B2 and Primesep D mixed-mode HPLC columns to retain and separate phosphate and sulfate ions. Both ions are retained by ion-exchange mechanism. Detection technique for analysis of these anions is Evaporative Light-Scattering Detector (ELSD).
Application Column
Primesep B2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSulfate
HPLC Separation of Citric Acid and Phosphate Ions in HILIC Chromatography
July 16, 2009
Citric acid and phosphate ion are separated by HILIC on Primesep N HILIC HPLC column. Both compounds are very hydrophilic. Citric acid and phosphate ions are well separated with excellent peak shape. Method can be used for quantitation of citrate and phosphate in various formulations. Because of the lack of UV activity, separation can be monitored by LC/MS, ELSD or Corona CAD. Method can be used for other hydrophilic organic and inorganic acids.
Application Column
Primesep N
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPhosphate
HPLC Separation of Sulfate and Phosphate Ions on Mixed-Mode HPLC
December 6, 2007
Sulfate and phosphate are separated on Primesep B2 and Primesep D column by anion-exchange mechanism. Because both sulfate and phosphate ions are not UV-active, ELSD is used to monitor separation of both anions. Method can be used to quantitate phosphate and sulfate in various pharmaceutical and chemical formulation, products and solution, drinking and ground water. Retention time is adjusted by increase or decrease of buffer concentrations. Two anions can be retained and separated on a very short column due to strong ion-exchange interaction. Method shows good reproducibility and versatility.
| Column | Primesep B2, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Ions, Hydrophilic, Ionizable, Vitamin, Supplements |
| Analyzing Compounds | Sodium, Phosphate, Sulfate |
Application Column
Primesep B2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsSodium
Sulfate
