Ethylphosphonic Acid

Ethylphosphonic Acid structural formula

CAS Number6779-09-5
Molecular FormulaC2H7O3P
Molecular Weight110.050
InChI KeyGATNOFPXSDHULC-UHFFFAOYSA-N
LogP-0.772
Synonyms
  • Ethylphosphonic acid
  • Phosphonic acid, ethyl-
  • 6779-09-5
  • EINECS 229-839-2
  • Ethylphosphonate

Applications:

HPLC Separation of Glyphosate, Glyphosine, Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column

December 4, 2019

HPLC Method for Ethylphosphonic Acid, Methylphosphonic Acid, Glyphosate, Glyphosine, Methylenediphosphonic acid, Phosphate, Phosphoric Acid on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylphosphonic Acid, Methylphosphonic Acid, Glyphosate, Glyphosine, Methylenediphosphonic acid, Phosphate, Phosphoric Acid.

Glyphosate is an herbicide with a chemical formula of C3H8NO5P. It works through blocking enzymes, like 5-enolpyruvylshikimate-3-phosphate synthase, essential for plant growth. It is typically found in agricultural work, but can occasionally be found in forestry and garden care.

Glyphosine is a synthetic plant growth regulator with the chemical formula of C4H11NO8P2. It is a colorless liquid that works through decreasing chlorophyll production.

Ethylphosphonic acid has a C2H5P(O)(OH)2 chemical formula. It is typically found as white crystals or crystalline powder. It is often used as an internal standard when researching fosfomycin in human plasma as well as a synthetic nucleotide analog.

Methylphosphonic acid is an organophosphorus compound with the chemical formula CH3P(OH)2. It is often used in some lubricant additives, textile treatments, and in synthesis of phosphonate compounds, like the previously mentioned Glyphosate.

Methylenediphosphonic acid has the chemical formula CH2[P(O)(OH)2]2. It is typically seen as a precursor in synthesis of Mesoporous aluminum organophosphate, if alkyltrimethylammonium, and Tetraester of methylenediphosphonic acids.

Phosphoric acid is an inorganic compound with chemical formula H3PO4. It is odorless and colorless, which leads to it’s common use in soft drunks to help preserve the product. It is also used in fertilizers, metal treatment, and corrosion inhibition. Excessive intake of it is not recommended.

Ethylphosphonic Acid, Methylphosphonic Acid, Glyphosate, Glyphosine, Methylenediphosphonic acid, Phosphate, Phosphoric Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.

ColumnNewcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN Gradient  -40-10%, 15 min
BufferFormic Acid Gradient  -1- 5%, 15 min
Flow Rate1.0 ml/min
DetectionCAD
Class of CompoundsAcids, Plant growth regulator, Herbicide, Hydrophilic, Ionizable
Analyzing CompoundsEthylphosphonic Acid, Methylphosphonic Acid, Glyphosate, Glyphosine, Methylenediphosphonic acid, Phosphate, Phosphoric Acid

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Ethylphosphonic Acid
Glyphosate
Glyphosine
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate
Phosphoric Acid

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Glyphosate, Ethylphosphonic Acid and Methylphosphonic Acid on Newcrom B Column

December 2, 2019

 HPLC Method for Methylphosphonic Acid, Ethylphosphonic Acid, Glyphosate, Phosphate on Newcrom B by SIELC Technologies

HPLC Separation of Glyphosate, Ethylphosphonic Acid and Methylphosphonic Acid on Newcrom B Column_1203 High Performance Liquid Chromatography (HPLC) Method for Analysis of Methylphosphonic Acid, Ethylphosphonic Acid, Glyphosate, Phosphate.

 

Ethylphosphonic acid has a C2H5P(O)(OH)2 chemical formula. It is typically found as white crystals or crystalline powder. It is often used as an internal standard when researching fosfomycin in human plasma as well as a synthetic nucleotide analog. Glyphosate is an herbicide with a chemical formula of C3H8NO5P. It works through blocking enzymes, like 5-enolpyruvylshikimate-3-phosphate synthase, essential for plant growth. It is typically found in agricultural work, but can occasionally be found in forestry and garden care.

 

Methylenediphosphonic acid has the chemical formula CH2[P(O)(OH)2]2. It is typically seen as a precursor in synthesis of Mesoporous aluminum organophosphate, if alkyltrimethylammonium, and Tetraester of methylenediphosphonic acids.

Phosphoric acid is an inorganic compound with chemical formula H3PO4. It is odorless and colorless, which leads to it’s common use in soft drunks to help preserve the product. It is also used in fertilizers, metal treatment, and corrosion inhibition. Excessive intake of it is not recommended.

 

Methylphosphonic Acid, Ethylphosphonic Acid, Glyphosate, Phosphate can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.

Condition

Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 40/59%
Buffer Formic Acid – 1%
Flow Rate 1.0 ml/min
Detection CAD

Description

Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Methylphosphonic Acid, Ethylphosphonic Acid, Glyphosate, Phosphate

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Ethylphosphonic Acid
Glyphosate
Methylphosphonic Acid
Phosphate

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column

December 2, 2019

HPLC Method for Ethylphosphonic Acid, Methylphosphonic Acid, Methylenediphosphonic acid, Phosphate, Phosphoric Acid on Newcrom B by SIELC Technologies HPLC Separation of Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric acids on Newcrom B Column_1201 High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylphosphonic Acid, Methylphosphonic Acid, Methylenediphosphonic acid, Phosphate, Phosphoric Acid

 

Ethylphosphonic acid has a C2H5P(O)(OH)2 chemical formula. It is typically found as white crystals or crystalline powder. It is often used as an internal standard when researching fosfomycin in human plasma as well as a synthetic nucleotide analog. Glyphosate is an herbicide with a chemical formula of C3H8NO5P. It works through blocking enzymes, like 5-enolpyruvylshikimate-3-phosphate synthase, essential for plant growth. It is typically found in agricultural work, but can occasionally be found in forestry and garden care.

Methylphosphonic acid is an organophosphorus compound with the chemical formula CH3P(OH)2. It is often used in some lubricant additives, textile treatments, and in synthesis of phosphonate compounds, like the previously mentioned Glyphosate.

Methylenediphosphonic acid has the chemical formula CH2[P(O)(OH)2]2. It is typically seen as a precursor in synthesis of Mesoporous aluminum organophosphate, if alkyltrimethylammonium, and Tetraester of methylenediphosphonic acids. Ethylphosphonic Acid, Methylphosphonic Acid, Methylenediphosphonic acid, Phosphate, Phosphoric Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.

Condition

Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 20/78%
Buffer Formic Acid – 2%
Flow Rate 1.0 ml/min
Detection CAD

Description

Class of Compounds Acids, Hydrophilic, Ionizable
Analyzing Compounds Ethylphosphonic Acid, Methylphosphonic Acid, Methylenediphosphonic acid, Phosphate, Phosphoric Acid

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Ethylphosphonic Acid
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate
Phosphoric Acid

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Effect of pH on Separation of Glyphosate and Ethylphosphonic Acid

July 8, 2011

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Glyphosate is broad spectrum herbicide to kill weeds. Presence of glyphosate in water, fruits and veggies is regulated. Alkylphosphonic acids are decomposition byproducts of chemical warfare agents. Analysis of phosphonic acid in water is regulated by Environmental Protection Agency (EPA). All phosphonic acids are polar acidic compounds. Method was developed for glyphosate and ethylphosphonic acid. Both compounds are retained by ion-exchange mechanism. Method is compatible with LC/MS and can be used for analysis of glyphosate and alkylphosphonic acids in various matrices.

 

Condition

Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 10%
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD 50C

 

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Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Glyphosate, Ethylphosphonic acid

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Ethylphosphonic Acid
Glyphosate

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Methylphosphonic Acid and Related Products

October 14, 2010


Alkylphosphonic acids are decomposition byproducts of chemical warfare agents. Analysis of phosphonic acid in water is regulated by Environmental Protection Agency (EPA). All phosphonic acids are polar acidic compounds. Analysis for retention and separation of methylphosphonic, ethylphosphonic, ethyl methylphosphonic and isopropyl methylphosphonic acids was developed using Primesep SB reversed-phase anion-exchange column. Method is compatible with LC/MS and can be used for quantitation of phosphonic acid in water.

Condition

Column Primesep SB, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD

 

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Description

Class of Compounds
 Acid,  Hydrophilic, Ionizable
Analyzing Compounds Methylphosphonic, Ethylphosphonic, Ethyl methylphosphonic, Isopropyl methylphosphonic acids

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Ethyl Methylphosphonic Acid
Ethylphosphonic Acid
Isopropyl Methylphosphonic Acid
Methylphosphonic Acid

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.