Effect of pH on Separation of Glyphosate and Ethylphosphonic Acid

chr_295.gif Glyphosate is broad spectrum herbicide to kill weeds. Presence of glyphosate in water, fruits and veggies is regulated. Alkylphosphonic acids are decomposition byproducts of chemical warfare agents. Analysis of phosphonic acid in water is regulated by Environmental Protection Agency (EPA). All phosphonic acids are polar acidic compounds. Method was developed for glyphosate and ethylphosphonic acid. Both compounds are retained by ion-exchange mechanism. Method is compatible with LC/MS and can be used for analysis of glyphosate and alkylphosphonic acids in various matrices.  


Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 10%
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD 50C


Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Glyphosate, Ethylphosphonic acid
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Ethylphosphonic Acid

Application Detection:
ELSD/MS Detection