HPLC Separation of Methylphosphonic Acid and Related Products


Alkylphosphonic acids are decomposition byproducts of chemical warfare agents. Analysis of phosphonic acid in water is regulated by Environmental Protection Agency (EPA). All phosphonic acids are polar acidic compounds. Analysis for retention and separation of methylphosphonic, ethylphosphonic, ethyl methylphosphonic and isopropyl methylphosphonic acids was developed using Primesep SB reversed-phase anion-exchange column. Method is compatible with LC/MS and can be used for quantitation of phosphonic acid in water.

Condition
Column Primesep SB, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD

 

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Description
Class of Compounds
  Acid,  Hydrophilic, Ionizable
Analyzing Compounds Methylphosphonic, Ethylphosphonic, Ethyl methylphosphonic, Isopropyl methylphosphonic acids

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Ethyl Methylphosphonic Acid
Ethylphosphonic Acid
Isopropyl Methylphosphonic Acid
Methylphosphonic Acid
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.