HPLC Separation of Methylphosphonic Acid and Related Products

Alkylphosphonic acids are decomposition byproducts of chemical warfare agents. Analysis of phosphonic acid in water is regulated by Environmental Protection Agency (EPA). All phosphonic acids are polar acidic compounds. Analysis for retention and separation of methylphosphonic, ethylphosphonic, ethyl methylphosphonic and isopropyl methylphosphonic acids was developed using Primesep SB reversed-phase anion-exchange column. Method is compatible with LC/MS and can be used for quantitation of phosphonic acid in water.


Column Primesep SB, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds  Acid,  Hydrophilic, Ionizable
Analyzing Compounds Methylphosphonic, Ethylphosphonic, Ethyl methylphosphonic, Isopropyl methylphosphonic acids

Application Analytes:
Ethyl Methylphosphonic Acid
Ethylphosphonic Acid
Isopropyl Methylphosphonic Acid
Methylphosphonic Acid