| CAS Number | 1984-15-2 |
|---|---|
| Molecular Formula | CH6O6P2 |
| Molecular Weight | 176 |
| InChI Key | MBKDYNNUVRNNRF-UHFFFAOYSA-N |
| LogP | -3.3 |
| Synonyms |
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Applications:
HPLC Separation of Glyphosate, Glyphosine, Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column
December 4, 2019
HPLC Method for Ethylphosphonic Acid, Methylphosphonic Acid, Glyphosate, Glyphosine, Methylenediphosphonic acid, Phosphate, Phosphoric Acid on Newcrom B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylphosphonic Acid, Methylphosphonic Acid, Glyphosate, Glyphosine, Methylenediphosphonic acid, Phosphate, Phosphoric Acid.
Glyphosate is an herbicide with a chemical formula of C3H8NO5P. It works through blocking enzymes, like 5-enolpyruvylshikimate-3-phosphate synthase, essential for plant growth. It is typically found in agricultural work, but can occasionally be found in forestry and garden care.
Glyphosine is a synthetic plant growth regulator with the chemical formula of C4H11NO8P2. It is a colorless liquid that works through decreasing chlorophyll production.
Ethylphosphonic acid has a C2H5P(O)(OH)2 chemical formula. It is typically found as white crystals or crystalline powder. It is often used as an internal standard when researching fosfomycin in human plasma as well as a synthetic nucleotide analog.
Methylphosphonic acid is an organophosphorus compound with the chemical formula CH3P(OH)2. It is often used in some lubricant additives, textile treatments, and in synthesis of phosphonate compounds, like the previously mentioned Glyphosate.
Methylenediphosphonic acid has the chemical formula CH2[P(O)(OH)2]2. It is typically seen as a precursor in synthesis of Mesoporous aluminum organophosphate, if alkyltrimethylammonium, and Tetraester of methylenediphosphonic acids.
Phosphoric acid is an inorganic compound with chemical formula H3PO4. It is odorless and colorless, which leads to it’s common use in soft drunks to help preserve the product. It is also used in fertilizers, metal treatment, and corrosion inhibition. Excessive intake of it is not recommended.
Ethylphosphonic Acid, Methylphosphonic Acid, Glyphosate, Glyphosine, Methylenediphosphonic acid, Phosphate, Phosphoric Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN Gradient -40-10%, 15 min |
| Buffer | Formic Acid Gradient -1- 5%, 15 min |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Acids, Plant growth regulator, Herbicide, Hydrophilic, Ionizable |
| Analyzing Compounds | Ethylphosphonic Acid, Methylphosphonic Acid, Glyphosate, Glyphosine, Methylenediphosphonic acid, Phosphate, Phosphoric Acid |
Application Column
Newcrom B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Glyphosate
Glyphosine
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate
Phosphoric Acid
HPLC Separation of Ethylphosphonic, Methylphosphonic, Methylenediphosphonic and Phosphoric Acids on Newcrom B Column
December 2, 2019
HPLC Method for Ethylphosphonic Acid, Methylphosphonic Acid, Methylenediphosphonic acid, Phosphate, Phosphoric Acid on Newcrom B by SIELC Technologies 
High Performance Liquid Chromatography (HPLC) Method for Analysis of Ethylphosphonic Acid, Methylphosphonic Acid, Methylenediphosphonic acid, Phosphate, Phosphoric Acid
Ethylphosphonic acid has a C2H5P(O)(OH)2 chemical formula. It is typically found as white crystals or crystalline powder. It is often used as an internal standard when researching fosfomycin in human plasma as well as a synthetic nucleotide analog. Glyphosate is an herbicide with a chemical formula of C3H8NO5P. It works through blocking enzymes, like 5-enolpyruvylshikimate-3-phosphate synthase, essential for plant growth. It is typically found in agricultural work, but can occasionally be found in forestry and garden care.
Methylphosphonic acid is an organophosphorus compound with the chemical formula CH3P(OH)2. It is often used in some lubricant additives, textile treatments, and in synthesis of phosphonate compounds, like the previously mentioned Glyphosate.
Methylenediphosphonic acid has the chemical formula CH2[P(O)(OH)2]2. It is typically seen as a precursor in synthesis of Mesoporous aluminum organophosphate, if alkyltrimethylammonium, and Tetraester of methylenediphosphonic acids. Ethylphosphonic Acid, Methylphosphonic Acid, Methylenediphosphonic acid, Phosphate, Phosphoric Acid can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 20/78% |
| Buffer | Formic Acid – 2% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Acids, Hydrophilic, Ionizable |
| Analyzing Compounds | Ethylphosphonic Acid, Methylphosphonic Acid, Methylenediphosphonic acid, Phosphate, Phosphoric Acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Methylenediphosphonic acid
Methylphosphonic Acid
Phosphate
Phosphoric Acid
