HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column

Separation type: Liquid Chromatography Mixed-mode





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HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column_1202




High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosate and Phosphate Ion


 
Glyphosate is a broad-spectrum herbicide which is used to kill weeds. The presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Glyphosate is characterized by its carboxylic acid and phosphate group on either end.
Glyphosate and phosphate ions can be retained and separated with a Newcrom B mixed-mode column using a mobile phase consisting of acetonitrile (MeCN), water, and formic acid (H3PO4) as a buffer. This method is compatible with mass spectrometry and be detected via a Charged aerosol detector (CAD).




 

Condition
Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 20%
Buffer Formic Acid – 0.5%
Flow Rate 1.0 ml/min
Detection CAD

 

Description
Class of Compounds
  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Phosphate, Glyphosate

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Glyphosate
Phosphate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.