HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column

Separation type: Liquid Chromatography Mixed-mode

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HPLC Separation of Glyphosate and Phosphate Ion on Newcrom B Column_1202

High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosate and Phosphate Ion
  Glyphosate is a broad-spectrum herbicide which is used to kill weeds. The presence of glyphosate is strongly regulated by various governing agencies in the US, Europe, and Asia. Glyphosate is characterized by its carboxylic acid and phosphate group on either end. Glyphosate and phosphate ions can be retained and separated with a Newcrom B mixed-mode column using a mobile phase consisting of acetonitrile (MeCN), water, and formic acid (H3PO4) as a buffer. This method is compatible with mass spectrometry and be detected via a Charged aerosol detector (CAD).

 

Condition

Column Newcrom B, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN - 20%
Buffer Formic Acid - 0.5%
Flow Rate 1.0 ml/min
Detection CAD
 

Description

Class of Compounds  Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Phosphate, Glyphosate

Application Analytes:
Glyphosate
Phosphate