Iodine


Iodine

CAS Number7553-56-2
Molecular FormulaI2
Molecular Weight253.809
InChI KeyPNDPGZBMCMUPRI-UHFFFAOYSA-N
LogP1.86
Synonyms
  • Iodine
  • 7553-56-2
  • Actomar
  • DentaPure DP 90
  • Diatomic iodine
  • Diiodine
  • Iodel FD
  • Iodine colloidal
  • Iodine crystals
  • Iodine molecule
  • Iodine sublimed
  • IODINE TINCTURE
  • Iosan Superdip
  • JOD, FREIES
  • Jodosan
  • Molecular iodine
  • NSC 42355
  • Tegodyne
  • Caswell No. 501
  • EINECS 231-442-4
  • EPA Pesticide Chemical Code 046905
  • Iodine ((127)I2)
  • Iodine Tincture USP
  • Vistarin
  • UNII-9679TC07X4
  • I2
  • Iode
  • Iodine-molecule
  • Iodio
  • Iodum
  • Jod
  • Jood
  • Tincture iodine
  • diiodane
  • 24503-90-0
  • 8012-81-5
  • 8012-85-9
  • 8031-47-8

Applications:

HPLC Separation of Inorganic Anions on Newcrom BH Column

October 23, 2019

HPLC Method for Sodium, Phosphate, Chloride, Bromide, Nitrate, Sulfate, Iodine, Perchlorate, Iodide on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium, Phosphate, Chloride, Bromide, Nitrate, Sulfate, Iodine, Perchlorate, Iodide 

Sodium, which  has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

Phosphate, PO43-, is a compound that plays an important part in biological energy transfer. It contains phosphorus, which is also a key component in bones and teeth. It is also a buffer, which helps maintain pH levels in a body.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

Bromide is typically used to refer to a compound or molecule that contains a bromide anion: Br. It is often found in anticonvulsants, flame-retardant materials, and cell stains.

Nitrate, NO3, is a compound of nitrogen and oxygen. It is essential as a nutrient in plants; therefore, it is often used in fertilizers. It is easily found in leafy greens. While it is important for cardiovascular health, too high of exposure to it can be dangerous.

Iodide, the Ionic form of Iodine, I is essential for thyroid hormone production. Lack of it can lead to iodine deficiency disorders. It is typically found in Iodized salt and occasionally as an antiseptic.

Sulfate is an ionic chemical with the formula SO42-. In nature, it is usually found in geodes like gypsum. It is also created in atmosphere from sulfur dioxide. It is used for a variety of applications, but most notably, coloring wood and creating light-reflecting paints.

Perchlorate is an inorganic compound with the formula ClO4. It is most typically used for explosives. For example, fireworks and rocket propellants. Due to it’s water solubility, it can easily contaminate it. When ingested, it can pose a health risk and cause developmental harm.

Sodium, Phosphate, Chloride, Bromide, Nitrate, Sulfate, Iodine, Perchlorate, Iodide can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Acetate buffer. Detection is performed using CAD.

ColumnNewcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 10/90%
BufferGradient AmAc pH 5.0 –  20-90 mM , 20 min
Flow Rate1.0 ml/min
DetectionCAD (Corona)  (MS-compatible mobile phase)
Class of CompoundsIons, Hydrophilic, Ionizable
Analyzing CompoundsSodium, Phosphate, Chloride, Bromide, Nitrate, Sulfate, Iodine, Perchlorate, Iodide

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Bromide
Chloride
Iodide
Iodine
Nitrate
Perchlorate
Phosphate
Sodium
Sulfate

Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Iodide and Thyroid Hormones

October 30, 2018

HPLC Method for Iodine, Methimazole, Levothyroxine (T4), 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine dihydrate (DIT), 3-Iodo-L-tyrosine (MIT), Liothyronine (T3), Iodide on Primesep D by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Iodine, Methimazole, Levothyroxine (T4), 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine dihydrate (DIT), 3-Iodo-L-tyrosine (MIT), Liothyronine (T3), Iodide 

Iodine is an important element in production of thyroid hormones, T3 and T4, which are essential regulators of organism’s metabolism. The glands’ concentration of Iodide, hormones T3 and T4, along with their precursors: 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine (DIT), and 3-Iodo-L-tyrosine (MIT) can now be studied simultaneously with this HPLC method.  In order to analyze the hydrophobic hormones along with iodide (a very polar inorganic ion) a special mixed-mode column, Primesep D, was used.  This column provides both strong hydrophobic and anion exchange properties.  The Primesep D silica-bonded ligand is comprised of a long alkyl chain and an embedded amino functional group.  The TFA was used as an ionic modifier to provide stable acidic pH and sufficient ion strength for the mobile phase.  The mobile phase composition is suitable for UV, MS, ELSD, and CAD detection.

ColumnPrimesep D, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN
BufferTFA
Flow Rate1.0 ml/min
DetectionUV, 230 nm
Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing CompoundsIodine, Methimazole, Levothyroxine (T4), 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine dihydrate (DIT), 3-Iodo-L-tyrosine (MIT), Liothyronine (T3), Iodide

Application Column

Primesep D

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
3,5-Diiodo-L-thyronine (T2)
3,5-Diiodo-L-tyrosine dihydrate (DIT)
3-Iodo-L-tyrosine (MIT)
Iodide
Iodine
Levothyroxine (T4)
Liothyronine (T3)
Methimazole

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Sodium, Chloride, Bromide, and Iodine

November 21, 2010


Halide ions are usually analyzed by ion-chromatography with a conductivity detector. Mixed-mode chromatography in combination with ELSD is offering a valuable alternative to ion-chromatography with conductivity detection. Chloride, bromide and iodide were separated on a Primesep SB mixed-mode anion-exchange column. Mobile phase is compatible with ELSD and LC/MS.

Condition

Column Primesep SB, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Hydrophilic, Ions
Analyzing Compounds Chloride, Sodium, Bromide, Iodine

 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Bromide
Chloride
Iodine
Sodium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.