Levothyroxine (T4)

Levothyroxine

 

Levothyroxine Synthetic form of the thyroid hormone T4

CAS Number51-48-9
Molecular Weight776.87
Synonyms
  • 3,3,5,5-Tetraiodo-L-thyronine, 3-[4-(4-Hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-L-alanine, L-Thyroxine, T4

Applications:

UV-Vis Spectrum of Thyroxine

July 23, 2024
UV-Vis Spectrum of Thyroxine. Absorption Maxima: 212 nm, 224 nm, 302 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Levothyroxine (T4)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Iodide and Thyroid Hormones

October 30, 2018

 

Iodine is an important element in production of thyroid hormones, T3 and T4, which are essential regulators of organism’s metabolism. The glands’ concentration of Iodide, hormones T3 and T4, along with their precursors: 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine (DIT), and 3-Iodo-L-tyrosine (MIT) can now be studied simultaneously with this HPLC method.  In order to analyze the hydrophobic hormones along with iodide (a very polar inorganic ion) a special mixed-mode column, Primesep D, was used.  This column provides both strong hydrophobic and anion exchange properties.  The Primesep D silica-bonded ligand is comprised of a long alkyl chain and an embedded amino functional group.  The TFA was used as an ionic modifier to provide stable acidic pH and sufficient ion strength for the mobile phase.  The mobile phase composition is suitable for UV, MS, ELSD, and CAD detection.

 

Condition

Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 230 nm

 

Description

Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing Compounds T3, T4, 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine (DIT), and 3-Iodo-L-tyrosine (MIT)

 

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
3,5-Diiodo-L-thyronine (T2)
3,5-Diiodo-L-tyrosine dihydrate (DIT)
3-Iodo-L-tyrosine (MIT)
Iodide
Iodine
Levothyroxine (T4)
Liothyronine (T3)
Methimazole
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Levothyroxine Sodium Tablets

June 11, 2018

HPLC Method for Analysis of Levothyroxine Sodium Tablets

 

Condition

Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer TFA – 0.1 %
Flow Rate 1.0 ml/min
Detection UV, 230 nm

 

Description

Class of Compounds
Drug, Hormone, Acid, Hydrophobic
Analyzing Compounds Levothyroxine

 

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Levothyroxine (T4)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.