3-Iodo-L-tyrosine (MIT)

CAS Number70-78-0
Molecular FormulaC9H10INO3
Molecular Weight307.087
InChI KeyUQTZMGFTRHFAAM-ZETCQYMHSA-N
LogP-1.1
Synonyms
  • MIT
  • H-Tyr(3-I)-OH
  • 3-IODOTYROSINE
  • 3-IODO-TYROSINE
  • (S)-2-Amino-3-(4-hydroxy-3-iodophenyl)propanoic acid
  • L-Tyrosine, 3-iodo-
  • Monoiodotyrosine
  • 3-Monoiodo-L-tyrosine
  • IODOTYROSINE
  • 3-Iodo-4-hydroxyphenylalanine
  • H-3-Iodo-Tyr-OH
  • (2S)-2-amino-3-(4-hydroxy-3-iodophenyl)propanoic acid
  • UNII-FRQ98U4U27
  • Tyrosine, 3-iodo-
  • MLS000069664
  • FRQ98U4U27
  • CHEBI:27847
  • UQTZMGFTRHFAAM-ZETCQYMHSA-N
  • AK-86417

Applications:

HPLC Method for Analysis of Iodide and Thyroid Hormones

October 30, 2018

HPLC Method for Iodine, Methimazole, Levothyroxine (T4), 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine dihydrate (DIT), 3-Iodo-L-tyrosine (MIT), Liothyronine (T3), Iodide on Primesep D by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Iodine, Methimazole, Levothyroxine (T4), 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine dihydrate (DIT), 3-Iodo-L-tyrosine (MIT), Liothyronine (T3), Iodide 

Iodine is an important element in production of thyroid hormones, T3 and T4, which are essential regulators of organism’s metabolism. The glands’ concentration of Iodide, hormones T3 and T4, along with their precursors: 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine (DIT), and 3-Iodo-L-tyrosine (MIT) can now be studied simultaneously with this HPLC method.  In order to analyze the hydrophobic hormones along with iodide (a very polar inorganic ion) a special mixed-mode column, Primesep D, was used.  This column provides both strong hydrophobic and anion exchange properties.  The Primesep D silica-bonded ligand is comprised of a long alkyl chain and an embedded amino functional group.  The TFA was used as an ionic modifier to provide stable acidic pH and sufficient ion strength for the mobile phase.  The mobile phase composition is suitable for UV, MS, ELSD, and CAD detection.

ColumnPrimesep D, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN
BufferTFA
Flow Rate1.0 ml/min
DetectionUV, 230 nm
Class of Compounds
Drug,  Hydrophilic, Ionizable, Supplements
Analyzing CompoundsIodine, Methimazole, Levothyroxine (T4), 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine dihydrate (DIT), 3-Iodo-L-tyrosine (MIT), Liothyronine (T3), Iodide

Application Column

Primesep D

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
3,5-Diiodo-L-thyronine (T2)
3,5-Diiodo-L-tyrosine dihydrate (DIT)
3-Iodo-L-tyrosine (MIT)
Iodide
Iodine
Levothyroxine (T4)
Liothyronine (T3)
Methimazole

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.