| CAS Number | 20461-54-5 |
|---|---|
| Molecular Formula | I- |
| Molecular Weight | 126.9045 |
| InChI Key | XMBWDFGMSWQBCA-UHFFFAOYSA-M |
| LogP | 2.9 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Iodide on Primesep B Column
January 31, 2023
HPLC Method for Analysis of Iodide on Primesep B by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Iodide
Condition
| Column | Primesep B, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 228 nm |
| Peak Retention Time | 4.61 min |
Description
| Class of Compounds | Inorganic |
| Analyzing Compounds | Iodide |
Application Column
Primesep B
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Method for Analysis of Inorganic anions on BIST™A+ Column
July 7, 2022
| Separation type: Bridge Ion Separation Technology, or BIST™ | ||||||||||||||
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| High Performance Liquid Chromatography (HPLC) Method for Analysis of Inorganic anions, Chloride, Bromide, Nitrate, Iodide, Perchlorate
Using SIELC’s newly introduced BIST™ method, a mixture of many different inorganic anions can be separated on a negatively-charged, cation-exchange BIST™ A column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry. |
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Application Column
Chloride
Iodide
Methanesulfonic Acid
Nitrate
Perchlorate
TFA (Trifluoroacetic Acid)
HPLC Determination of Iodide on Primesep SB
March 31, 2021
| Column | Primesep SB, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | HClO4 – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 228 nm |
| Class of Compounds |
Ion |
| Analyzing Compounds | Iodide |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
HPLC Separation of Inorganic Anions on Newcrom BH Column
October 23, 2019
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | Gradient AmAc pH 5.0 – 20-90 mM , 20 min |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |
| Class of Compounds | Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Phosphate, Chloride, Bromide, Nitrate, Iodide, Sulfate, Perchlorate |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsChloride
Iodide
Iodine
Nitrate
Perchlorate
Phosphate
Sodium
Sulfate
HPLC Method for Analysis of Iodide and Thyroid Hormones
October 30, 2018
Iodine is an important element in production of thyroid hormones, T3 and T4, which are essential regulators of organism’s metabolism. The glands’ concentration of Iodide, hormones T3 and T4, along with their precursors: 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine (DIT), and 3-Iodo-L-tyrosine (MIT) can now be studied simultaneously with this HPLC method. In order to analyze the hydrophobic hormones along with iodide (a very polar inorganic ion) a special mixed-mode column, Primesep D, was used. This column provides both strong hydrophobic and anion exchange properties. The Primesep D silica-bonded ligand is comprised of a long alkyl chain and an embedded amino functional group. The TFA was used as an ionic modifier to provide stable acidic pH and sufficient ion strength for the mobile phase. The mobile phase composition is suitable for UV, MS, ELSD, and CAD detection.
| Column | Primesep D, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | TFA |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Drug, Hydrophilic, Ionizable, Supplements |
| Analyzing Compounds | T3, T4, 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine (DIT), and 3-Iodo-L-tyrosine (MIT) |
Application Column
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options3,5-Diiodo-L-tyrosine dihydrate (DIT)
3-Iodo-L-tyrosine (MIT)
Iodide
Iodine
Levothyroxine (T4)
Liothyronine (T3)
Methimazole
