HPLC Method for Iodide on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Iodide.
Iodide is an ion with the symbol I–. There is currently a worldwide iodine deficiency, which is a leading preventable cause of intellectual disability, has caused some governments to mandate iodized salt to be sold. It is naturally occurring as a mineral, Iodargyrite.
Iodide can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a perchloric acid buffer. Detection is performed using UV.
| Column | Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | HClO4 – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 228 nm |
| Class of Compounds |
Ion |
| Analyzing Compounds | Iodide |
Application Column
Primesep SB
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended




