HPLC Method for Analysis of Iodide on Primesep B by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Iodide is an ion with the symbol I–. There is currently a worldwide iodine deficiency, which is a leading preventable cause of intellectual disability, has caused some governments to mandate iodized salt to be sold. It is naturally occurring as a mineral, Iodargyrite.
Iodide can be retained and analyzed using the [mode] stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a perchloric acid buffer. Detection is performed using UV.
You can find detailed UV spectra of Iodide and information about its various lambda maxima by visiting the following link.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Iodide
Condition
| Column | Primesep B, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | UV 228 nm |
| Peak Retention Time | 4.61 min |
Description
| Class of Compounds | Inorganic |
| Analyzing Compounds | Iodide |
Application Column
Primesep B
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
