HPLC Method for Separation Povidone-iodine (PVP-I) on PVP Column

 

HPLC Method for Iodide, PVP on PVP by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Iodide, PVP

Polidone Iodide (Povidone-Iodine / PVP-I)
Polidone Iodide, also known as Povidone-Iodine (PVP-I), is a widely used antiseptic made from iodine and polyvinylpyrrolidone (PVP). This combination allows for a slow, sustained release of iodine, enhancing antimicrobial effects while reducing irritation.

PVP (Polyvinylpyrrolidone): A water-soluble synthetic polymer that acts as:

  • Stabilizer
  • Emulsifier
  • Solubilizer
  • Protective colloid

It helps form a stable complex with iodine, improving its medical use.

Iodine: The active antimicrobial agent, effective against:

  • Bacteria
  • Viruses
  • Fungi
  • Protozoa

Slow release from the PVP complex ensures long-lasting, gentle antiseptic action.

Iodide, PVP can be retained and analyzed using the PVP column. The analysis utilizes an step gradient method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium acetate. Detection is performed using ELSD.

Column PVP, 2.1 x 50 mm, 5 µm, 100 A, dual ended
Mobile Phase Step gradient MeCN/H2O
Buffer Ammonium Acetate  pH 4.0 –  20 mM
Flow Rate 0.4 ml/min
Detection ELSD
LOD PVP 2 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
Class of Compounds Polymer
Analyzing Compounds Iodide, PVP

Application Column

PVP

Column Diameter: 2.1 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Iodide
PVP
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.