TFA (Trifluoroacetic Acid)

CAS Number76-05-1
Molecular FormulaC2HF3O2
Molecular Weight114.023
InChI KeyDTQVDTLACAAQTR-UHFFFAOYSA-N
LogP0.766
Synonyms
  • Acetic acid, trifluoro-
  • Trifluoroacetic acid
  • Acetic acid, 2,2,2-trifluoro-
  • 76-05-1
  • 4-02-00-00458
  • 2,2,2-Trifluoroacetic acid
  • Acetic acid, 2,2,2-trifluoro-
  • Acide trifluoroacetique
  • acido trifluoroacetico
  • NSC 77366
  • Perfluoroacetic acid
  • TRIFLUORESSIGSAEURE
  • Trifluoressigsaure
  • trifluoroacetic acid
  • Trifluoroethanoic acid
  • UN 2699
  • BRN 0742035
  • EINECS 200-929-3
  • Trifluoracetic acid
  • Kyselina trifluoroctova
  • UNII-E5R8Z4G708

Applications:

HPLC Method for Analysis of Inorganic anions on BIST™A+ Column

2022-07-07

 

Separation type: Bridge Ion Separation Technology, or BIST™
 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Inorganic anions, Chloride, Bromide, Nitrate, Iodide,  Perchlorate

Using SIELC’s newly introduced BIST™ method, a mixture of many different inorganic anions can be separated on a negatively-charged, cation-exchange BIST™ A column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry.

Condition

Column BIST™ A+, 4.6×50 mm, 5µm, 100A
Mobile Phase MeCN – 90%
Buffer TMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) formate – 5 mM pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD, 70C

 

Description

Class of Compounds
 Acid, Inorganic anion
Analyzing Compounds Chloride, Bromide, Nitrate, Iodide,  Perchlorate, TFA, Methanesulfonic acid
Application Analytes:
Bromide
Chloride
Iodide
Methanesulfonic Acid
Nitrate
Perchlorate
TFA (Trifluoroacetic Acid)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

BIST A

SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.

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BIST A+

SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.

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HPLC Method for Analysis of Fluoroacetic acid, Difluoroacetic acid and Trifluoroacetic acid on BIST A+ Column

2022-07-07

 

Separation type: Bridge Ion Separation Technology, or BIST™
 

HPLC Method for Analysis of Fluoroacetic acid, Difluoroacetic acid and Trifluoroacetic acid on BIST A+ Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Fluoroacetic acid, Difluoroacetic acid and Trifluoroacetic acid

Condition

Column BIST A+, 2.1×100 mm, 3 µm, 100A
Mobile Phase MeCN – 90%
Buffer TMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) formate – 5 mM pH 4.0
Flow Rate 0.4 ml/min
Detection Conductivity

 

Description

Class of Compounds
Acids
Analyzing Compounds Fluoroacetic acid, Difluoroacetic acid and Trifluoroacetic acid
Application Analytes:
Difluoroacetic acid
Fluoroacetic acid
TFA (Trifluoroacetic Acid)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

BIST A+

SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns currently on the market! When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.

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HPLC Separation of Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid,   Trichloroacetic acid

2020-05-20


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Acetic acid and its substitution derivatives, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, difluoroacetic acid and trifluoroacetic acid, are heavily used as building blocks for more complex compounds in organic synthesis. All six acids can be isocratically separated in HPLC using Newcrom BH mixed-mode column with acetonitrile (ACN) and water mobile phase with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.

Condition

Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid
Analyzing Compounds Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid (TFA),   Trichloroacetic acid
Application Analytes:
Acetic Acid
Chloroacetic acid
Dichloroacetic acid
Difluoroacetic acid
TFA (Trifluoroacetic Acid)
Trichloroacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Newcrom BH

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 columns are standard reverse phase columns with octyldecyl silane chains (C18) attached to porous silica. We have also designed a line of Newcrom R1 columns with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).

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HPLC Separation of Haloacetic acids on Newcrom BH Column

2020-05-20


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Condition

Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid
Analyzing Compounds Dichloroacetic acid, Trifluoroacetic acid (TFA),   Trichloroacetic acid
Application Analytes:
Dichloroacetic acid
TFA (Trifluoroacetic Acid)
Trichloroacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Newcrom BH

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 columns are standard reverse phase columns with octyldecyl silane chains (C18) attached to porous silica. We have also designed a line of Newcrom R1 columns with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).

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HPLC Determination of TFA on Newcrom BH Column

2020-05-19


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Trifluoroacetic acid (TFA) is a substitution derivative of acetic acid, with fluorine atoms replacing the hydrogen atoms of the acetyl group. It is also a much stronger acid than acetic acid. TFA is widely used in organic synthesis as well as ion pairing reagent or buffer in HPLC. TFA can be detected at low UV. Using Newcrom BH mixed-mode column, a mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer can be used to retain TFA and UV detect it at 200nm.

Condition

Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid
Analyzing Compounds Trifluoroacetic acid (TFA)
Application Analytes:
TFA (Trifluoroacetic Acid)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Newcrom BH

The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 columns are standard reverse phase columns with octyldecyl silane chains (C18) attached to porous silica. We have also designed a line of Newcrom R1 columns with a new outer design. This column and corresponding adapter entirely eliminate the need for any high-pressure fittings or tubing as well as minimizing all possible dead volumes. Furthermore, if a leak ever occurs in the high-pressure column inlet, the mobile phase is contained within the column adapter (no external leakage).

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HPLC Analysis of Trifluoroacetic and Acetic Acid on Obelisc N Mixed-Mode HPLC Column

2015-05-11

Separation type: Liquid Chromatography Mixed-mode








Trifluoroacetic acid (TFA) and acetic acid (AcOH) are very commonly used in organic chemistry. They were separated using an Obelisc N column which uses HILIC/anion-exchange. The mixed-mode of Obelisc N allows for better peak shape and retention of carboxylic acids. Sulfuric acid was used to enhance polar retention by suppressing ionization of the strong acid TFA.

 



Condition

Column Obelisc N, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 60%
Buffer H2SO4 – 0.05%
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description

Class of Compounds
 Acid, Hydrophilic, Ionizable
Analyzing Compounds Trifluoroacetic acid (TFA), Acetic acid (AcOH)

 

Application Analytes:
Acetic Acid
TFA (Trifluoroacetic Acid)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Obelisc N

SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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HPLC Separation of Inorganic Anions

2010-11-21


Organic and inorganic acids and ions can be separated on a Primesep B4 column based on their ionic properties. Method can be used for quantitation of residual acids in various products and sample matrices. Trifluoracetic, hydrochloric, methanesulfonic, and nitric acids are separated using ACN-water-ammonium formate. Ions can be detected by ELSD, CAD or LC/MS.

Condition

Column Primesep B4, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Ions, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Phosphate, Chloride,  Nitrate, Sulfate, Iodide, Perchlorate, Trifluoracetic

 

Application Analytes:
Hydrochloric Acid
Methanesulfonic Acid
Nitrate
Nitric Acid
Organic Acids
Perchloric Acid
TFA (Trifluoroacetic Acid)

Application Detection:
ELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep B4

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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HPLC Analysis of Components of Ionic Liquids by Mixed-Mode Chromatography

2008-09-14

Ionic liquid is an ionic compound which is liquid at room (or close to room) temperature. Most of the ionic liquids are in a dynamic equilibrium where at any time more than 99.99% of the liquid is made up of ionic, rather than molecular, species. Room-temperature ionic liquids consist of bulky cation (for example, substituted imidazolium) compounds. A wide range of anions is used as counter ions in ionic liquids: organic and inorganic anions such as chloride, iodide, tetrafluoroborate, hexafluorophosphate, bistriflimide, triflate, tosylate. Ionic liquids are widely used as solvents in organic reactions. When products are isolated from ionic liquids, they need to be analyzed for residual ionic liquid content.
Because both constituents of the ionic liquid are very different in terms of charge and hydrophobic properties, it is impossible to analyze entire ionic liquids by traditional chromatography. An effective and universal method for analysis of ionic liquids is developed on an Obelisc R HPLC column. Components on the ionic liquids are retained based on ionic and hydrophobic interactions. Obelisc R column has both positively and negatively charged ionic groups, making it possible to retain and separate cations and anions of ionic liquids on one column. Method can be used for quantitative of various ionic liquids containing organic and inorganic ions. Retention time of basic component can be effectively adjusted by pH, stronger anionic and hydrophobic counter-ions might require higher buffer concentration. Composition can be monitored by combination of UV and ELSD or by LC/MS.

Condition

Column Obelisc R , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc 
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Acid
Analyzing Compounds 1-Methyl-3-propylimidazolium, Bromide Ion, Methylsulfonic Acid, Trifluoroacetic Acid, Perchloric Acid, p-Toluenesulfonic Acid
Application Analytes:
1-Methyl-3-Propylimidazolium
Bromide
Ionic Liquid
Methylsulfonic Acid
Perchloric Acid
TFA (Trifluoroacetic Acid)
p-Toluenesulfonic Acid (PTSA)

Application Detection:
UV Detection
ELSD/MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Obelisc R

SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Retention of TFA on Atlantis and Obelisc N

2008-08-22

Trifluoroacetic acid (TFA) is a strong carboxylic acid, with pKa of 0.3. TFA is widely used in chemistry as reagent and as counter-ion for basic drugs and other compounds. TFA is very polar in nature, and has low UV activity. It is often required to quantitate trifluoracetic acid in various formulations. HPLC method for determination of trifluoroacetic acid on Obelisc N column provides a viable alternative for quantitation of TFA. TFA is retained by ion-exchange mechanism on mixed-mode HILIC column. Relative comparison shows high loadability of the method. Obelisc N columns show much better peak shape and retention than traditional HPLC columns (Atlantis T3 from Waters). Perfect peak symmetry is achieved by adjusting amount of acetonitrile, buffer pH and buffer concentration. TFA is monitored by low UV (200 nm). Alternatively trifluoroacetic acid can be analyzed by ESLD with ammonium formate in the mobile phase. TFA forms non-volatile salt with ammonia, thus giving ability to quantitate it.

Application Analytes:
TFA (Trifluoroacetic Acid)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Obelisc N

SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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HPLC Analysis of TFA

2008-07-05

Dual functionality of Obelisc N column allows to retain and provide efficient peaks of strong carboxylic acids such as TFA. Simple UV quantitation and identification method became possible due to the unique properties of this stationary phase. In case of an RP separation poor retention and peak symmetry was obtained at a very low concentration of ACN. Sulfuric acid was used to suppress ionization of TFA to increase the hydrophobicity. In case of a typical HILIC column the phosphoric acid was used to increase ionization to make TFA more polar to enhance polar retention. The types of columns that do not have the mixed mode nature of Obelisc N can’t provide significant retention and efficiency.

Application Analytes:
TFA (Trifluoroacetic Acid)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Obelisc N

SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options