HPLC Method for Analysis of TFA (Trifluoroacetic Acid) on Primesep B4 by SIELC Technologies
Trifluoroacetic Acid (TFA) is a synthetic organofluorine acid with the chemical formula C2HF3O2. It is a substitution derivative of acetic acid, with fluorine atoms replacing the hydrogen atoms of the acetyl group. It is also a much stronger acid than acetic acid. TFA is widely used in organic synthesis as well as ion pairing reagent or buffer in HPLC. It is also employed in the analysis of compounds by nuclear magnetic resonance (NMR) spectroscopy. It is corrosive and toxic to aquatic life and mammals, causing severe irritation and burns to skin, eyes, and the respiratory tract. Not only that, TFA is also highly mobile and persistent, leading to high retention of it in soil and water. Determination of it’s threat level on the environmental and health levels are still ongoing. You can find detailed UV spectra of Trifluoroacetic Acid and information about its various lambda maxima by visiting the following link.
TFA (Trifluoroacetic Acid) can be retained and analyzed using an Primesep B4 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD
| Column | Primesep B4, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | Ammonium formate pH 3.0 – 60 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Samples | 1 mg/ml |
| Injection volume | 3 µl |
| LOD* | 1 ppm |
| Class of Compounds | Quaternity amines |
| Analyzing Compounds | TFA (Trifluoroacetic Acid) |
Application Column
Primesep B4
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
