Acetic acid and its substitution derivatives, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, difluoroacetic acid and trifluoroacetic acid, are heavily used as building blocks for more complex compounds in organic synthesis. All six acids can be isocratically separated in HPLC using Newcrom BH mixed-mode column with acetonitrile (ACN) and water mobile phase with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.
Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 50/50% |
Buffer | H2SO4 – 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV 200nm |
Class of Compounds | Acid |
Analyzing Compounds | Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid (TFA), Trichloroacetic acid |
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
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