| CAS Number | 64-19-7 |
|---|---|
| Molecular Formula | C2H4O2 |
| Molecular Weight | 60.052 |
| InChI Key | QTBSBXVTEAMEQO-UHFFFAOYSA-N |
| LogP | -0.170 |
| Synonyms |
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Applications:
HPLC Method For Separation of Acetic Acid and Formic Acid on Newcrom BH Column
2021-09-22
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetic Acid, Formic Acid
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | H2O |
| Buffer | Gradient H3PO4 – 0.05-0.5%, 5 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV 205 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Acetic Acid, Formic Acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
Select optionsApplication Analytes:
Acetic AcidFormic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
HPLC Separation of Bromoacetic and Chloroacetic Acids on Newcrom BH Column
2020-06-17
Acetic acid and its various chloro- and bromo- forms are widely used in organic chemistry. Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns. By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer. UV detection at 200nm.
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 – 0.3% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Acetic acid, Chloroacetic acid, Bromoacetic acid, Difluoroacetic acid, Unknown, Dichloroacetic acid, Bromochloroacetic acid, Dibromoacetic acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
Select optionsApplication Analytes:
Acetic AcidBromoacetic acid
Bromochloroacetic acid
Chloroacetic acid
Dibromoacetic acid
Dichloroacetic acid
Difluoroacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
HPLC Separation of Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid, Trichloroacetic acid
2020-05-20
Acetic acid and its substitution derivatives, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, difluoroacetic acid and trifluoroacetic acid, are heavily used as building blocks for more complex compounds in organic synthesis. All six acids can be isocratically separated in HPLC using Newcrom BH mixed-mode column with acetonitrile (ACN) and water mobile phase with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid (TFA), Trichloroacetic acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
Select optionsApplication Analytes:
Acetic AcidChloroacetic acid
Dichloroacetic acid
Difluoroacetic acid
TFA (Trifluoroacetic Acid)
Trichloroacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
HPLC Analysis of Trifluoroacetic and Acetic Acid on Obelisc N Mixed-Mode HPLC Column
2015-05-11
Separation type: Liquid Chromatography Mixed-mode
Trifluoroacetic acid (TFA) and acetic acid (AcOH) are very commonly used in organic chemistry. They were separated using an Obelisc N column which uses HILIC/anion-exchange. The mixed-mode of Obelisc N allows for better peak shape and retention of carboxylic acids. Sulfuric acid was used to enhance polar retention by suppressing ionization of the strong acid TFA.
| Column | Obelisc N, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 60% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Trifluoroacetic acid (TFA), Acetic acid (AcOH) |
Application Column
Obelisc N
SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - Buffer concentration, Buffer pH, and Organic Modifier Concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsApplication Analytes:
Acetic AcidTFA (Trifluoroacetic Acid)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
HPLC Separation of Acetic acid and Acetic Hydrazide
2010-10-14
Acetic acid and acetic hydrazide are quantitated in one run using a Primesep N HILIC mixed-mode column. Method can be used for determination of acetic acid and acetic hydrazide by HPLC.
Application Column
Primesep N
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsApplication Analytes:
Acetic AcidAcetic Hydrazide
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
