Acetic acid and its various chloro- and bromo- forms are widely used in organic chemistry. Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns. By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer. UV detection at 200nm.
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 – 0.3% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Acetic acid, Chloroacetic acid, Bromoacetic acid, Difluoroacetic acid, Unknown, Dichloroacetic acid, Bromochloroacetic acid, Dibromoacetic acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsBromoacetic acid
Bromochloroacetic acid
Chloroacetic acid
Dibromoacetic acid
Dichloroacetic acid
Difluoroacetic acid
