HPLC Method for Difluoroacetic acid, Dichloroacetic acid, Dibromoacetic acid, Acetic Acid, Bromoacetic acid, Chloroacetic acid, Bromochloroacetic acid on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Difluoroacetic acid, Dichloroacetic acid, Dibromoacetic acid, Acetic Acid, Bromoacetic acid, Chloroacetic acid, Bromochloroacetic acid.
Acetic acid and its various chloro- and bromo- forms are widely used in organic chemistry. Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns. By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer. UV detection at 200nm.
Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN/H2O – 10/90% |
Buffer | H2SO4 – 0.3% |
Flow Rate | 1.0 ml/min |
Detection | UV 200nm |
Class of Compounds | Acid |
Analyzing Compounds | Difluoroacetic acid, Dichloroacetic acid, Dibromoacetic acid, Acetic Acid, Bromoacetic acid, Chloroacetic acid, Bromochloroacetic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Bromoacetic acid
Bromochloroacetic acid
Chloroacetic acid
Dibromoacetic acid
Dichloroacetic acid
Difluoroacetic acid