HPLC Separation of Bromoacetic and Chloroacetic Acids on Newcrom BH Column

HPLC.cloud View on hplc.cloud Acetic acid and its various chloro- and bromo- forms are widely used in organic chemistry.  Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns.  By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer.  UV detection at 200nm.


Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer H2SO4 - 0.3%
Flow Rate 1.0 ml/min
Detection UV 200nm


Class of Compounds Acid
Analyzing Compounds Acetic acid, Chloroacetic acid,  Bromoacetic acid, Difluoroacetic acid, Unknown, Dichloroacetic acid, Bromochloroacetic acid, Dibromoacetic acid

Application Analytes:
Acetic Acid
Bromoacetic acid
Bromochloroacetic acid
Chloroacetic acid
Dibromoacetic acid
Dichloroacetic acid
Difluoroacetic acid