HPLC Separation of Bromoacetic and Chloroacetic Acids on Newcrom BH Column

HPLC Method for Difluoroacetic acid, Dichloroacetic acid, Dibromoacetic acid, Acetic Acid, Bromoacetic acid, Chloroacetic acid, Bromochloroacetic acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Difluoroacetic acid, Dichloroacetic acid, Dibromoacetic acid, Acetic Acid, Bromoacetic acid, Chloroacetic acid, Bromochloroacetic acid.

Acetic acid and its various chloro- and bromo- forms are widely used in organic chemistry.  Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns.  By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer.  UV detection at 200nm.

Condition
Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 10/90%
Buffer H2SO4 – 0.3%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description
Class of Compounds Acid
Analyzing Compounds Difluoroacetic acid, Dichloroacetic acid, Dibromoacetic acid, Acetic Acid, Bromoacetic acid, Chloroacetic acid, Bromochloroacetic acid

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Acetic Acid
Bromoacetic acid
Bromochloroacetic acid
Chloroacetic acid
Dibromoacetic acid
Dichloroacetic acid
Difluoroacetic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.