CAS Number | 79-11-8 |
---|---|
Molecular Formula | C2H3ClO2 |
Molecular Weight | 94.490 |
InChI Key | FOCAUTSVDIKZOP-UHFFFAOYSA-N |
LogP | 0.22 |
Synonyms |
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Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
Chloroacetic acid is a popular building block in organic syntheses. Dichloroacetic acid and Trichloroacetic acid are frequently used in cosmetic treatments to remove tattos and in chemical peels, as well as topical medication for the ablation of warts. Using SIELC’s newly introduced BIST™ method, a mixture of these three aicds can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected with a Conductivity Detector.
Column | BIST™ A+, 2.1×100 mm, 3 µm, 100A |
Mobile Phase | MeCN – 90% |
Buffer | TMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) formate – 5 mM pH 4.0 |
Flow Rate | 0.4 ml/min |
Detection | Conductivity |
Class of Compounds | Acid, Carboxylic acid, Haloacetic acid |
Analyzing Compounds | Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid |
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select optionsAcetic acid and its various chloro- and bromo- forms are widely used in organic chemistry. Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns. By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer. UV detection at 200nm.
Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 10/90% |
Buffer | H2SO4 – 0.3% |
Flow Rate | 1.0 ml/min |
Detection | UV 200nm |
Class of Compounds | Acid |
Analyzing Compounds | Acetic acid, Chloroacetic acid, Bromoacetic acid, Difluoroacetic acid, Unknown, Dichloroacetic acid, Bromochloroacetic acid, Dibromoacetic acid |
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
Select options
Acetic acid and its substitution derivatives, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, difluoroacetic acid and trifluoroacetic acid, are heavily used as building blocks for more complex compounds in organic synthesis. All six acids can be isocratically separated in HPLC using Newcrom BH mixed-mode column with acetonitrile (ACN) and water mobile phase with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.
Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 50/50% |
Buffer | H2SO4 – 0.2% |
Flow Rate | 1.0 ml/min |
Detection | UV 200nm |
Class of Compounds | Acid |
Analyzing Compounds | Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid (TFA), Trichloroacetic acid |
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
Select optionsChloroacetic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
The Newcrom columns are a family of reverse phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse phase column with low silanol activity.
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