| CAS Number | 76-03-9 |
|---|---|
| Molecular Formula | C2HCl3O2 |
| Molecular Weight | 163.381 |
| InChI Key | YNJBWRMUSHSURL-UHFFFAOYSA-N |
| LogP | 1.33 |
| Synonyms |
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Applications:
HPLC MS Method for Analysis of Trichloroacetic acid (TCA) on BIST A+ Column
March 14, 2024
HPLC Method for Analysis of Trichloroacetic acid on BIST A+ Column by SIELC Technologies
Separation type: Liquid Chromatography Reversed-phase
Trichloroacetic acid (TCA) is a chemical compound with the formula CCl3COOH. It is a strong organic acid and a derivative of acetic acid, where three of the four hydrogen atoms in the methyl group of acetic acid are replaced by chlorine atoms.
Trichloroacetic acid is commonly used in various applications, including:
Chemical Synthesis: TCA serves as a reagent in various chemical reactions, such as the production of pharmaceuticals, dyes, and pesticides.
Protein Precipitation: In biochemistry and molecular biology, TCA is often used for the precipitation of proteins from solution. It is particularly useful for removing proteins from samples for further analysis.
Skin Care: TCA is also used in dermatology as a peeling agent to treat various skin conditions, including acne scars, sun damage, and wrinkles. It promotes exfoliation and the regeneration of skin cells.
DNA and RNA Precipitation: TCA is employed to precipitate nucleic acids (DNA and RNA) from solution, facilitating their isolation and purification in molecular biology experiments.
Trichloroacetic acid is corrosive and should be handled with care.
Trichloroacetic acid can be retained and analyzed on a BIST A+ column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a TMDAP formate as a buffer. This analysis method can be detected using LC MS.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Trichloroacetic acid on BIST A+ Column by SIELC Technologies
Condition
| Column | BIST A+, 2.1 x 100 mm, 3 µm, 100 A |
| Mobile Phase | MeCN/H2O – 90/10% |
| Buffer | TMDAP formate – 5 mM pH 4.0 |
| Flow Rate | 0.2 ml/min |
| Detection | SIM 161- |
| Sample | 0.1µl/ml |
| LOD* | 2 ppb |
Description
| Class of Compounds | Bis-biguanide |
| Analyzing Compounds | Trichloroacetic acid |
Application Column
HPLC Method for Analysis of Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid on BIST™ A+ Column
July 7, 2022
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid
Chloroacetic acid is a popular building block in organic syntheses. Dichloroacetic acid and Trichloroacetic acid are frequently used in cosmetic treatments to remove tattos and in chemical peels, as well as topical medication for the ablation of warts. Using SIELC’s newly introduced BIST™ method, a mixture of these three aicds can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected with a Conductivity Detector.
Condition
| Column | BIST™ A+, 2.1×100 mm, 3 µm, 100A |
| Mobile Phase | MeCN – 90% |
| Buffer | TMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) formate – 5 mM pH 4.0 |
| Flow Rate | 0.4 ml/min |
| Detection | Conductivity |
Description
| Class of Compounds | Acid, Carboxylic acid, Haloacetic acid |
| Analyzing Compounds | Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid |
Application Column
BIST A+
BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.
Select optionsDichloroacetic acid
Trichloroacetic acid
HPLC Separation of Bromoacetic and Chloroacetic acids on Newcrom B Column
September 10, 2020
Bromo- and chloro- acetic acids are widely used in organic chemistry. Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns. By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer.
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Acid |
| Analyzing Compounds | Bromoacetic acid, Dichloroacetic acid, Dibromoacetic acid, Trichloroacetic acid |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsDibromoacetic acid
Dichloroacetic acid
Trichloroacetic acid
HPLC Separation of Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid, Trichloroacetic acid
May 20, 2020
Acetic acid and its substitution derivatives, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, difluoroacetic acid and trifluoroacetic acid, are heavily used as building blocks for more complex compounds in organic synthesis. All six acids can be isocratically separated in HPLC using Newcrom BH mixed-mode column with acetonitrile (ACN) and water mobile phase with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid (TFA), Trichloroacetic acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsChloroacetic acid
Dichloroacetic acid
Difluoroacetic acid
TFA (Trifluoroacetic Acid)
Trichloroacetic acid
HPLC Separation of Haloacetic acids on Newcrom BH Column
May 20, 2020
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Dichloroacetic acid, Trifluoroacetic acid (TFA), Trichloroacetic acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsTFA (Trifluoroacetic Acid)
Trichloroacetic acid
Separation of Trichloroacetic acid on Newcrom R1 HPLC column
February 16, 2018
Trichloroacetic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options