Trichloroacetic acid

Trichloroacetic acid structural formula

CAS Number76-03-9
Molecular FormulaC2HCl3O2
Molecular Weight163.381
InChI KeyYNJBWRMUSHSURL-UHFFFAOYSA-N
LogP1.33
Synonyms
  • Trichloroacetic acid
  • Acetic acid, 2,2,2-trichloro-
  • 76-03-9
  • 4-02-00-00508
  • Acetic acid, trichloro-
  • Acetic acid, 2,2,2-trichloro-
  • Aceto-Caustin
  • Acide trichloroacetique
  • acido tricloroacetico
  • Amchem Grass Killer
  • NSC 215204
  • NSC 77363
  • TCA (acid)
  • Trichloracetic acid
  • TRICHLORESSIGSAEURE
  • Trichloressigsaure
  • Trichloroethanoic acid
  • UN 1839
  • UN 2564
  • BRN 0970119
  • Caswell No. 870
  • EINECS 200-927-2
  • EPA Pesticide Chemical Code 081002
  • Konesta
  • Trichloroacetate
  • Trichloromethanecarboxylic acid
  • Acide trichloracetique
  • Kyselina trichloroctova
  • Trichloorazijnzuur
  • UNII-5V2JDO056X
  • TCA

Applications:

HPLC MS Method for Analysis of Trichloroacetic acid (TCA) on BIST A+ Column

March 14, 2024

HPLC Method for Analysis of Trichloroacetic acid on BIST A+ Column by SIELC Technologies

Separation type: Liquid Chromatography Reversed-phase

HPLC Method for Analysis of Trichloroacetic acid on BIST A+ Column by SIELC Technologies

Trichloroacetic acid (TCA) is a chemical compound with the formula CCl3COOH. It is a strong organic acid and a derivative of acetic acid, where three of the four hydrogen atoms in the methyl group of acetic acid are replaced by chlorine atoms.

Trichloroacetic acid is commonly used in various applications, including:

Chemical Synthesis: TCA serves as a reagent in various chemical reactions, such as the production of pharmaceuticals, dyes, and pesticides.

Protein Precipitation: In biochemistry and molecular biology, TCA is often used for the precipitation of proteins from solution. It is particularly useful for removing proteins from samples for further analysis.

Skin Care: TCA is also used in dermatology as a peeling agent to treat various skin conditions, including acne scars, sun damage, and wrinkles. It promotes exfoliation and the regeneration of skin cells.

DNA and RNA Precipitation: TCA is employed to precipitate nucleic acids (DNA and RNA) from solution, facilitating their isolation and purification in molecular biology experiments.

Trichloroacetic acid is corrosive and should be handled with care.

Trichloroacetic acid can be retained and analyzed on a BIST A+ column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a TMDAP formate as a buffer. This analysis method can be detected using LC MS.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Trichloroacetic acid on BIST A+ Column by SIELC Technologies

Condition

ColumnBIST A+, 2.1 x 100 mm, 3 µm, 100 A
Mobile PhaseMeCN/H2O – 90/10%
BufferTMDAP formate – 5 mM pH 4.0
Flow Rate0.2 ml/min
DetectionSIM 161-
Sample0.1µl/ml
LOD*2 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsBis-biguanide
Analyzing CompoundsTrichloroacetic acid

Application Column

BIST A+

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A

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Application Analytes:
Trichloroacetic acid

Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid on BIST™ A+ Column

July 7, 2022

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of  Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid on BIST™ A+ Column
HPLC Method for Analysis of Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid on BIST™ A+ Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Chloroacetic acid, Dichloroacetic acid and Trichloroacetic acid

Chloroacetic acid is a popular building block in organic syntheses. Dichloroacetic acid and Trichloroacetic acid are frequently used in cosmetic treatments to remove tattos and in chemical peels, as well as topical medication for the ablation of warts. Using SIELC’s newly introduced BIST™ method, a mixture of these three aicds can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected with a Conductivity Detector.

Condition

ColumnBIST™ A+, 2.1×100 mm, 3 µm, 100A
Mobile PhaseMeCN – 90%
BufferTMDAP ( N,N,N’,N’-Tetramethyl-1,3-diaminopropane) formate – 5 mM pH 4.0
Flow Rate0.4 ml/min
DetectionConductivity

Description

Class of CompoundsAcid, Carboxylic acid, Haloacetic acid
Analyzing CompoundsChloroacetic acid, Dichloroacetic acid and Trichloroacetic acid

Application Column

BIST A+

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

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Application Analytes:
Chloroacetic acid
Dichloroacetic acid
Trichloroacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Bromoacetic and Chloroacetic acids on Newcrom B Column

September 10, 2020


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Bromo- and chloro- acetic acids are widely used in organic chemistry.  Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns.  By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer.

Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection CAD

Description

Class of Compounds Acid
Analyzing Compounds Bromoacetic acid, Dichloroacetic acid,  Dibromoacetic acid, Trichloroacetic acid

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Bromoacetic acid
Dibromoacetic acid
Dichloroacetic acid
Trichloroacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid, Trichloroacetic acid

May 20, 2020


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Acetic acid and its substitution derivatives, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, difluoroacetic acid and trifluoroacetic acid, are heavily used as building blocks for more complex compounds in organic synthesis. All six acids can be isocratically separated in HPLC using Newcrom BH mixed-mode column with acetonitrile (ACN) and water mobile phase with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.

Condition

Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid
Analyzing Compounds Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid (TFA),   Trichloroacetic acid

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Acetic Acid
Chloroacetic acid
Dichloroacetic acid
Difluoroacetic acid
TFA (Trifluoroacetic Acid)
Trichloroacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Haloacetic acids on Newcrom BH Column

May 20, 2020


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Condition

Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 200nm

Description

Class of Compounds Acid
Analyzing Compounds Dichloroacetic acid, Trifluoroacetic acid (TFA),   Trichloroacetic acid

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Dichloroacetic acid
TFA (Trifluoroacetic Acid)
Trichloroacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Trichloroacetic acid on Newcrom R1 HPLC column

February 16, 2018
Separation of Trichloroacetic acid on Newcrom C18 HPLC column

Trichloroacetic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Trichloroacetic acid
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.