HPLC Method for Bromoacetic acid, Dibromoacetic acid, Dichloroacetic acid, Trichloroacetic acid on Newcrom B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bromoacetic acid, Dibromoacetic acid, Dichloroacetic acid, Trichloroacetic acid.
Bromo– and chloro– acetic acids are widely used in organic chemistry. Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns. By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer.
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds | Acid |
| Analyzing Compounds | Bromoacetic acid, Dibromoacetic acid, Dichloroacetic acid, Trichloroacetic acid |
Application Column
Newcrom B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Bromoacetic acidDibromoacetic acid
Dichloroacetic acid
Trichloroacetic acid
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
