| CAS Number | 64-18-6 |
|---|---|
| Molecular Formula | CH2O2 |
| Molecular Weight | 46.026 |
| InChI Key | BDAGIHXWWSANSR-UHFFFAOYSA-N |
| LogP | -0.540 |
| Synonyms |
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Applications:
HPLC Method for Simultaneous Determination of Formamide, Propionic Acid and Formic Acid on Newcrom BH Column
August 24, 2022
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Formamide, Propionic Acid, Formic Acid
Formamide is a popular industrial solvent with a wide variety of applications, including in the manufacturing of drugs, herbicides, pesticides, as a paper and fiber softener, and as a solvent for a plethora of ionic compounds. Propionic acid is a popular antifungal and antibacterial compound commonly used in food production as a preservative. Formic Acid is a very popular organic acid with a plethora of industrial applications, including as an agricultural preservative, a key component in fuel cells, and as an ionic modifier in HPLC analysis. These 3 organic acids can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
| Column | Newcrom BH, 4.6×250 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 10 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Formamide, Propionic Acid, Formic Acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsFormic acid
Propionic acid
HPLC Method For Separation of Acetic Acid and Formic Acid on Newcrom BH Column
September 22, 2021
HPLC Method for Acetic Acid, Formic acid on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetic Acid, Formic acid.
Acetic Acid is the second simplest carboxylic acid with the chemical formula CH3COOH. It is well known for being the active ingredient in vinegar, leading to the belief that it is the earliest mass produced acid, dating back to 3BC. Outside of it’s use in food and household matters, it is also used in production of vinyl acetate and cellulose acetate.
Formic Acid is the simplest carboxylic acid with the chemical formula CH2O2. It is naturally found in insects, weeds, fruits, and vegetables. It is used by insects as a method of self-defense. In agriculture, it is used as a preservative and antibacterial agent. In chromatography, it is used as a volatile pH modifier. It is used significantly in the tanning of leather, dyeing and finishing of textiles, and production of rubber.
Acetic Acid, Formic acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | H2O |
| Buffer | Gradient H3PO4 – 0.05-0.5%, 5 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV 205 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Acetic Acid, Formic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Formic acid
Separation of Formic acid on Newcrom R1 HPLC column
February 16, 2018
Formic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options