HPLC Method for Simultaneous Determination of Formamide, Propionic Acid and Formic Acid on Newcrom BH Column

HPLC Method for Formamide, Propionic acid, Formic acid on Newcrom BH by SIELC Technologies

HPLC Method for Simultaneous Determination of Formamide, Propionic Acid, Formic Acid on Newcrom BH

High Performance Liquid Chromatography (HPLC) Method for Analysis of Formamide, Propionic acid, Formic acid.

Formamide is a popular industrial solvent with the chemical formula CH3NO. It has a wide variety of applications, including in the manufacturing of drugs, herbicides, pesticides, as a paper and fiber softener, and as a solvent for a plethora of ionic compounds. Historically, it was produced by treating formic acid and ammonia.

Propionic acid is a popular antifungal and antibacterial compound with C3H6O2. It is an oily liquid with an unpleasant smell. Primarily, the acid is used in food production as a preservative.

Formic Acid is the simplest carboxylic acid with the chemical formula CH2O2. It is naturally found in insects, weeds, fruits, and vegetables. It is used by insects as a method of self-defense. In agriculture, it is used as a preservative and antibacterial agent. In chromatography, it is used as a volatile pH modifier. It is used significantly in the tanning of leather, dyeing and finishing of textiles, and production of rubber.

Formamide, Propionic acid, Formic acid can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.

Condition
Column Newcrom BH, 4.6 x 250 mm, 3 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.1%
Flow Rate 10 ml/min
Detection UV 210 nm

Description
Class of Compounds Acid, Hydrophilic
Analyzing Compounds Formamide, Propionic acid, Formic acid

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Formamide
Formic acid
Propionic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.