| CAS Number | 75-12-7 |
|---|---|
| Molecular Formula | CH3NO |
| Molecular Weight | 45.042 |
| InChI Key | ZHNUHDYFZUAESO-UHFFFAOYSA-N |
| LogP | -1.51 |
| Synonyms |
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Applications:
HPLC Method for Simultaneous Determination of Formamide, Propionic Acid and Formic Acid on Newcrom BH Column
August 24, 2022
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Formamide, Propionic Acid, Formic Acid
Formamide is a popular industrial solvent with a wide variety of applications, including in the manufacturing of drugs, herbicides, pesticides, as a paper and fiber softener, and as a solvent for a plethora of ionic compounds. Propionic acid is a popular antifungal and antibacterial compound commonly used in food production as a preservative. Formic Acid is a very popular organic acid with a plethora of industrial applications, including as an agricultural preservative, a key component in fuel cells, and as an ionic modifier in HPLC analysis. These 3 organic acids can be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 210 nm with high resolution and peak symmetry.
| Column | Newcrom BH, 4.6×250 mm, 3 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 10 ml/min |
| Detection | UV 210 nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Formamide, Propionic Acid, Formic Acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsFormic acid
Propionic acid
Separation of Formamide on Newcrom R1 HPLC column
May 16, 2018
Formamide can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options