HPLC Method For Separation of Acetic Acid and Formic Acid on Newcrom BH Column

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetic Acid, Formic Acid


Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase H2O
Buffer Gradient H3PO4 – 0.05-0.5%, 5 min
Flow Rate 1.0 ml/min
Detection UV 205  nm


Class of Compounds Acid
Analyzing Compounds Acetic Acid, Formic Acid


Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Acetic Acid
Formic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.