HPLC Method for Acetic Acid, Formic acid on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetic Acid, Formic acid.
Acetic Acid is the second simplest carboxylic acid with the chemical formula CH3COOH. It is well known for being the active ingredient in vinegar, leading to the belief that it is the earliest mass produced acid, dating back to 3BC. Outside of it’s use in food and household matters, it is also used in production of vinyl acetate and cellulose acetate.
Formic Acid is the simplest carboxylic acid with the chemical formula CH2O2. It is naturally found in insects, weeds, fruits, and vegetables. It is used by insects as a method of self-defense. In agriculture, it is used as a preservative and antibacterial agent. In chromatography, it is used as a volatile pH modifier. It is used significantly in the tanning of leather, dyeing and finishing of textiles, and production of rubber.
Acetic Acid, Formic acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | H2O |
| Buffer | Gradient H3PO4 – 0.05-0.5%, 5 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV 205 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Acetic Acid, Formic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Formic acid
