| CAS Number | 64-19-7 |
|---|---|
| Molecular Formula | C2H4O2 |
| Molecular Weight | 60.052 |
| InChI Key | QTBSBXVTEAMEQO-UHFFFAOYSA-N |
| LogP | -0.170 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Dicarbon Carboxylic Acids on Newcrom BH Column
June 6, 2023
HPLC Method for Analysis of Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid on Newcrom BH by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
- Glyoxylic Acid (CHO-COOH): This is the smallest α-keto acid. It is a yellowish solid that occurs naturally and is useful industrially. It participates in many biochemical reactions. Its reduced form, D-lactic acid, can be found in various microorganisms.
- Glycolic Acid (HOCH2COOH): This is the smallest α-hydroxy acid (AHA). It’s derived from sugar cane and is a colorless, odorless, and hygroscopic crystalline solid. It is used in various skin-care products because of its capability to penetrate skin.
- Acetic Acid (CH3COOH): It is a simple carboxylic acid. It’s an important chemical reagent and industrial chemical, primarily used in the production of cellulose acetate for photographic film and polyvinyl acetate for wood glue, as well as synthetic fibers and fabrics. It gives vinegar its sour taste and pungent smell.
- Oxalic Acid (C2H2O4): This is a colorless crystalline solid that forms a colorless solution in water. It occurs naturally in many foods, but too much can lead to kidney stones. It’s used in many different industrial processes, including rust removal, bleaching, and as a reducing agent in developing photographic film.
These four acids be separate and analyzed on a reverse-phase Newcrom BH, 4.6 x 150 mm, 3 µm, 100 A column with a mobile phase consisting of water and perchloric acid as a buffer modifier. This analysis method can be UV detected at 200 nm.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid
Condition
| Column | Newcrom BH, 4.6 x 150 mm, 3 µm, 100 A |
| Mobile Phase | H2O -99.7% |
| Buffer | HClO4 – 0.3 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
Description
| Class of Compounds | Dicarbon Carboxylic Acids |
| Analyzing Compounds | Oxalic Acid, Glycolic acid, Acetic Acid, Glyoxylic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Glycolic acid
Glyoxylic acid
Oxalic Acid
HPLC Method For Separation of Acetic Acid and Formic Acid on Newcrom BH Column
September 22, 2021
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetic Acid, Formic Acid
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | H2O |
| Buffer | Gradient H3PO4 – 0.05-0.5%, 5 min |
| Flow Rate | 1.0 ml/min |
| Detection | UV 205 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Acetic Acid, Formic Acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsFormic acid
HPLC Separation of Bromoacetic and Chloroacetic Acids on Newcrom BH Column
June 17, 2020
Acetic acid and its various chloro- and bromo- forms are widely used in organic chemistry. Their similar structure makes the acids difficult to retain and separate on reverse-phase HPLC columns. By using Newcrom BH mixed-mode column which also has ion-exchange properties, the separation can be achieved with a simple isocratic method and relatively short time with a mobile phase of acetonitrile (ACN), water and sulfuric acid (H2SO4) buffer. UV detection at 200nm.
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H2SO4 – 0.3% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Acetic acid, Chloroacetic acid, Bromoacetic acid, Difluoroacetic acid, Unknown, Dichloroacetic acid, Bromochloroacetic acid, Dibromoacetic acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsBromoacetic acid
Bromochloroacetic acid
Chloroacetic acid
Dibromoacetic acid
Dichloroacetic acid
Difluoroacetic acid
HPLC Separation of Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid, Trichloroacetic acid
May 20, 2020
Acetic acid and its substitution derivatives, chloroacetic acid, dichloroacetic acid, trichloroacetic acid, difluoroacetic acid and trifluoroacetic acid, are heavily used as building blocks for more complex compounds in organic synthesis. All six acids can be isocratically separated in HPLC using Newcrom BH mixed-mode column with acetonitrile (ACN) and water mobile phase with sulfuric acid (H2SO4) as buffer and UV detected at 200nm.
| Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Acetic acid, Chloroacetic acid, Dichloroacetic acid, Trifluoroacetic acid (TFA), Trichloroacetic acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsChloroacetic acid
Dichloroacetic acid
Difluoroacetic acid
TFA (Trifluoroacetic Acid)
Trichloroacetic acid
HPLC Analysis of Trifluoroacetic and Acetic Acid on Obelisc N Mixed-Mode HPLC Column
May 11, 2015
Separation type: Liquid Chromatography Mixed-mode
Trifluoroacetic acid (TFA) and acetic acid (AcOH) are very commonly used in organic chemistry. They were separated using an Obelisc N column which uses HILIC/anion-exchange. The mixed-mode of Obelisc N allows for better peak shape and retention of carboxylic acids. Sulfuric acid was used to enhance polar retention by suppressing ionization of the strong acid TFA.
| Column | Obelisc N, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 60% |
| Buffer | H2SO4 – 0.05% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Trifluoroacetic acid (TFA), Acetic acid (AcOH) |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsTFA (Trifluoroacetic Acid)
HPLC Separation of Acetic acid and Acetic Hydrazide
October 14, 2010
Acetic acid and acetic hydrazide are quantitated in one run using a Primesep N HILIC mixed-mode column. Method can be used for determination of acetic acid and acetic hydrazide by HPLC.
Application Column
Primesep N
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsAcetic Hydrazide
