HPLC Determination of TFA on Newcrom BH Column

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Trifluoroacetic acid (TFA) is a substitution derivative of acetic acid, with fluorine atoms replacing the hydrogen atoms of the acetyl group. It is also a much stronger acid than acetic acid. TFA is widely used in organic synthesis as well as ion pairing reagent or buffer in HPLC. TFA can be detected at low UV. Using Newcrom BH mixed-mode column, a mobile phase of acetonitrile (ACN) and water with sulfuric acid (H2SO4) buffer can be used to retain TFA and UV detect it at 200nm.


Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 200nm


Class of Compounds Acid
Analyzing Compounds Trifluoroacetic acid (TFA)

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
TFA (Trifluoroacetic Acid)
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