HPLC Separation of Inorganic Anions


Organic and inorganic acids and ions can be separated on a Primesep B4 column based on their ionic properties. Method can be used for quantitation of residual acids in various products and sample matrices. Trifluoracetic, hydrochloric, methanesulfonic, and nitric acids are separated using ACN-water-ammonium formate. Ions can be detected by ELSD, CAD or LC/MS.

Condition

Column Primesep B4, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Ions, Hydrophilic, Ionizable
Analyzing Compounds Sodium, Phosphate, Chloride,  Nitrate, Sulfate, Iodide, Perchlorate, Trifluoracetic

 

Application Column

Primesep B4

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Hydrochloric Acid
Methanesulfonic Acid
Nitrate
Nitric Acid
Organic Acids
Perchloric Acid
TFA (Trifluoroacetic Acid)

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.