| CAS Number | 75-75-2 |
|---|---|
| Molecular Formula | CH4O3S |
| Molecular Weight | 96.1 |
| InChI Key | AFVFQIVMOAPDHO-UHFFFAOYSA-N |
| LogP | -0.9 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Inorganic anions on BIST™A+ Column
2022-07-07
| Separation type: Bridge Ion Separation Technology, or BIST™ | ||||||||||||||
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| High Performance Liquid Chromatography (HPLC) Method for Analysis of Inorganic anions, Chloride, Bromide, Nitrate, Iodide, Perchlorate
Using SIELC’s newly introduced BIST™ method, a mixture of many different inorganic anions can be separated on a negatively-charged, cation-exchange BIST™ A column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these anions can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry. |
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Application Column
BIST A
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select options
BIST A+
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select optionsApplication Analytes:
BromideChloride
Iodide
Methanesulfonic Acid
Nitrate
Perchlorate
TFA (Trifluoroacetic Acid)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
HPLC Method for Analysis of Sulfonic acids on BIST A+ Column
2022-06-16
Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies
HPLC Method for Analysis of Sulfonic acids on BIST A+ Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sulfonic acid
Methanesulfonic acid is a popular non-volatile catalyst used in organic reactions due to it being a strong acid. Other sulfonic acids, like 1-Pentanesulfonic acid, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, and 1=Dodecanesulfonic acid are typically used in ion chromatography and for organic syntheses. Using SIELC’s newly introduced BIST™ method, a mixture of these Sulfonic acids can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these Sulfonic acids can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry (LC-MS) and CAD.
Condition
| Column | BIST™ A+, 2.1×100 mm, 3 µm, 100A |
| Mobile Phase | MeCN – 90% |
| Buffer | TMDAP formate pH 4.0 – 5,0 mM |
| Flow Rate | 0.4 ml/min |
| Detection | ELSD, 80C |
Description
| Class of Compounds | Acid, Sulfonic acid |
| Analyzing Compounds | 1-Dodecanesulfonic acid, 1-Decanesulfonic acid, 1-Heptanesulfonic Acid, 1-Pentanesulfonic acid, Methanesulfonic acid |
Application Column
BIST A+
SIELC Technologies’ BIST™ Columns are a new and simple way to achieve many separations that are traditionally difficult or impossible to achieve with any other HPLC columns. When used with our BIST™ mobile phases, these ion exchange columns can generate very strong retention of analytes that have the same charge polarity as the stationary phase, unlocking new chromatography applications that were previously too difficult to achieve.
Select optionsApplication Analytes:
1-Decanesulfonic acid1-Dodecanesulfonic acid
1-Heptanesulfonic acid
1-Pentanesulfonic acid
Methanesulfonic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
HPLC Separation of Organic Acids Glucuronic Acid, Methanesulfonic Acid, and Butanesulfonic Acid on Primesep SB Column
2015-05-11
The organic acids glucuronic acid, methanesulfonic acid, and butanesulfonic acid were separated using Primesep SB mixed-mode column. Primesep SB is a reverse-phase column with embedded basic ion-pairing groups.
| Column | Primesep SB, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | Gradient AmFm pH 3.0- 2-50 mM, 3 min, 3 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Glucuronic acid, Methanesulfonic acid, Butanesulfonic acid |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsApplication Analytes:
Benzenesulfonic AcidButanesulfonic acid
Glucuronic acid
Methanesulfonic Acid
Application Detection:
ELSD DetectionUV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
HPLC Separation of Inorganic Anions
2010-11-21
Organic and inorganic acids and ions can be separated on a Primesep B4 column based on their ionic properties. Method can be used for quantitation of residual acids in various products and sample matrices. Trifluoracetic, hydrochloric, methanesulfonic, and nitric acids are separated using ACN-water-ammonium formate. Ions can be detected by ELSD, CAD or LC/MS.
| Column | Primesep B4, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Phosphate, Chloride, Nitrate, Sulfate, Iodide, Perchlorate, Trifluoracetic |
Application Column
Primesep B4
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsApplication Analytes:
Hydrochloric AcidMethanesulfonic Acid
Nitrate
Nitric Acid
Organic Acids
Perchloric Acid
TFA (Trifluoroacetic Acid)
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
HPLC Separation of Potassium, Perchlorate, Methanesulfonic, Chloride, Bromide, and Nitrate Ions on Obelisc N
2010-03-03
Ion chromatography is usually used for analysis of hydrophilic organic and inorganic ions. Same separation can be achieved on HILIC/mixed-mode Obelisc N HPLC columns. Obelisc N HPLC columns have very polar groups on their surface: one of the groups is basic and the other acidic. In case of low organic concentration, two groups are connected by hydrophilic linker. Obelisc N column can be used as cation-exchange and anion-exchange column. This allows to separate positively and negatively charged molecules in one run. Five anions (chloride, bromide, methanesulfonate, nitrate and perchlorate) along with one cation (sodium) were separated in one run. Method is compatible with ELSD, CAD and LC/MS and can be used for analysis of various hydrophilic and hydrophobic cations and anions in one HPLC run.
| Column | Obelisc N, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Hydrophilic, Ions |
| Analyzing Compounds | Chloride, Nitrate, Chlorate, Bromide, Potassium |
Application Column
Obelisc N
SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - Buffer concentration, Buffer pH, and Organic Modifier Concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsApplication Analytes:
BromideChloride
Methanesulfonic Acid
Nitrate
Nitric Acid
Potassium
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
Separation of Sulphonium Ions in Polar Organic Mode
2005-10-04
The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both polar organic and ion-exchange modes in the separation of sulfonium ions. The high organic mobile phase retains methane sulfonic acid by a polar organic mechanism and separates methoxydimethylsulphonium and trimethylsulfphonium cations by polar organic and cation exchange modes. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN), and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD).
| Column | Primesep 100, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 35/65% |
| Buffer | TFA |
| Flow Rate | 1.5 ml/min |
| Detection | ELSD |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sulphonium Ions, Methoxydimethylsulphonium, Trimethylsulfphonium |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select options
Primesep C
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select optionsApplication Analytes:
Methanesulfonic AcidMethoxydimethylsulphonium
Sulfonium Ions
Trimethylsulfonium
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
