1-Dodecanesulfonic acid

CAS Number1510-16-3
Molecular FormulaC12H26O3S
Molecular Weight250.40
  • 1510-16-3
  • dodecane-1-sulfonic acid
  • Dodecane-1-sulphonic acid
  • Dodecylsulfonic acid


HPLC Method for Analysis of Sulfonic acids on BIST A+ Column

June 16, 2022

Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

HPLC Method for Analysis of Sulfonic acids on BIST A+ Column by SIELC Technologies

HPLC Method for Analysis of Sulfonic acids on BIST A+ Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Sulfonic acid

Methanesulfonic acid is a popular non-volatile catalyst used in organic reactions due to it being a strong acid. Other sulfonic acids, like 1-Pentanesulfonic acid, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, and 1=Dodecanesulfonic acid are typically used in ion chromatography and for organic syntheses. Using SIELC’s newly introduced BIST™ method, a mixture of these Sulfonic acids can be separated on a negatively-charged, cation-exchange BIST™ A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these Sulfonic acids can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry (LC-MS) and CAD.


ColumnBIST™ A+, 2.1×100 mm, 3 µm, 100A
Mobile PhaseMeCN – 90%
BufferTMDAP formate pH 4.0 – 5,0 mM
Flow Rate0.4 ml/min
DetectionELSD, 80C


Class of CompoundsAcid, Sulfonic acid
Analyzing Compounds1-Dodecanesulfonic acid, 1-Decanesulfonic acid, 1-Heptanesulfonic Acid, 1-Pentanesulfonic acid, Methanesulfonic acid

Application Column


BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
1-Decanesulfonic acid
1-Dodecanesulfonic acid
1-Heptanesulfonic acid
1-Pentanesulfonic acid
Methanesulfonic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.