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For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

HPLC Method for Separation of Hydrophobic, Cationic and Anionic Surfactants on Newcrom BH by SIELC Technologies

Surfactants, also known as surface-active agents, are compounds that lower the surface tension (or interfacial tension) between two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents, or dispersants.

They are often classified according to the charge of the polar head group:

Anionic Surfactants: These surfactants have a negative charge on their polar head group. Common examples include soap, sodium laureth sulfate, and sodium lauryl sulfate. They are commonly used in detergents and shampoos due to their ability to emulsify oils and hold dirt in suspension, so it can be rinsed away.

Cationic Surfactants: These surfactants have a positive charge on their polar head group. Examples include cetyltrimethylammonium bromide (CTAB) and benzalkonium chloride. These are often used as antiseptics and can also be found in hair conditioners because they reduce static cling.

Nonionic Surfactants: These surfactants have no charge on their polar head group. Examples include alcohol ethoxylates, nonylphenol ethoxylates, and polysorbates. Nonionic surfactants are often used in laundry and dishwasher detergents.

Sodium, which has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

1-Ethylpyridinium bromide is an ionic liquid with the chemical formula C₇H₁₀BrN. It is primarily used as a solvent at temperatures below 100^oC catalysts, and electrolytes in , It has an ethyl group attached to a pyridinium ring, and its counterion is bromide (Br-). You can find detailed UV spectra of 1-Ethylpyridinium bromide and information about its various lambda maxima by visiting the following link.

Benzalkonium chloride, also known as Zephiran or Alkyldimethylbenzylammonium chloride, is a positively charged surfactant with the chemical formula C₆H₅CH₂N(CH₃)₂RCl. It is often used as a pharmaceutical preservative and as an antiseptic. You can find detailed UV spectra of Benzalkonium chloride and information about its various lambda maxima by visiting the following link.

Cetylpyridinium Chloride, also written as CPC, is a cationic quaternary ammonium compound with the chemical formula C₂₁H₃₈ClN. It is commonly used in mouthwashes, toothpastes, lozenges, throat sprays, breath sprays, and nasal sprays due to it’s antiseptic abilities. You can find detailed UV spectra of Cetylpyridinium Chloride and information about its various lambda maxima by visiting the following link.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl^–. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

1-Pentanesulfonic acid is an organosulfonic acid with the chemical formula C₅H₁₂O₃S. It is primarily used in it’s sodium salt form as an anionic pairing agent. You can find detailed UV spectra of 1-Pentanesulfonic acid and information about its various lambda maxima by visiting the following link.

Lauric acid, also known as dodecanoic acid, is a saturated fatty acid. Its chemical formula is CH₃(CH₂)₁₀COOH. It is white in color, and it is a solid at room temperature with a melting point of around 44 degrees Celsius (111 degrees Fahrenheit). It is relatively inexpensive and has a long shelf life, which makes it an attractive ingredient in various commercial products. Lauric acid is found naturally in various plant and animal fats and oils, but the largest amounts of lauric acid are found in coconut oil and palm kernel oil. In these oils, lauric acid can make up to 50% of the total fat content. It is commonly used in the manufacturing of soaps and cosmetics due to its ability to form a lather when mixed with water. It is also used in food for its role in digestion and metabolic processes. It’s a medium-chain fatty acid, which means it is more easily absorbed and utilized by the body compared to long-chain fatty acids. You can find detailed UV spectra of Dodecanoic acid (Lauric acid) and information about its various lambda maxima by visiting the following link.

p-Toluenesulfonic Acid, also known as tosylic acid, which are written as PTSA, pTSA, pTsOH, and TsOH, is an organic compound with the chemical formula C₇H₈O₃S. It is used primarily in organic synthesis. You can find detailed UV spectra of p-Toluenesulfonic Acid and information about its various lambda maxima by visiting the following link.

1-Octanesulfonic acid is an anionic organosulfonic acid with the chemical formula C₈H₁₈O₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Heptanesulfonic acid and information about its various lambda maxima by visiting the following link.

Sodium dodecyl sulfate (SDS), or sodium lauryl sulfate (SLS), is a surfactant used in cleaning, hygienic, and food products. It has a chemical formula C₁₂H₂₅NaSO₄, meaning it has twelve carbon hydrophobic tail attached to a polar sulfate. At low concentrations, it is used as a whipping aid and emulsifier in foods containing egg whites. It has a similar use in pharmaceutics as an ionic solubilizer and, also, an emulsifier. In laboratory uses, it is used as a component for lysing cells during RNA extraction or DNA extraction. You can find detailed UV spectra of Sodium dodecyl sulfate and information about its various lambda maxima by visiting the following link.

Benzalkonium chloride, Cetylpyridinium Chloride, 1-Pentanesulfonic acid, Dodecanoic acid (Lauric acid), p-Toluenesulfonic Acid (PTSA), 1-Octanesulfonic acid, Sodium dodecyl sulfate, 1-Ethylpyridinium bromide can be retained, separated, and analyzed using a reverse-phase [colimn] column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

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HPLC Method for Separation of Hydrophobic, Cationic, Nonionic and Anionic Surfactants on Newcrom BH by SIELC Technologies

Surfactants, also known as surface-active agents, are compounds that lower the surface tension (or interfacial tension) between two liquids or between a liquid and a solid. Surfactants may act as detergents, wetting agents, emulsifiers, foaming agents, or dispersants.

They are often classified according to the charge of the polar head group:

Anionic Surfactants: These surfactants have a negative charge on their polar head group. Common examples include soap, sodium laureth sulfate, and sodium lauryl sulfate. They are commonly used in detergents and shampoos due to their ability to emulsify oils and hold dirt in suspension, so it can be rinsed away.

Cationic Surfactants: These surfactants have a positive charge on their polar head group. Examples include cetyltrimethylammonium bromide (CTAB) and benzalkonium chloride. These are often used as antiseptics and can also be found in hair conditioners because they reduce static cling.

Nonionic Surfactants: These surfactants have no charge on their polar head group. Examples include alcohol ethoxylates, nonylphenol ethoxylates, and polysorbates. Nonionic surfactants are often used in laundry and dishwasher detergents.

Sodium, which  has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

Benzalkonium chloride, also known as Zephiran or Alkyldimethylbenzylammonium chloride, is a positively charged surfactant with the chemical formula C₆H₅CH₂N(CH₃)₂RCl. It is often used as a pharmaceutical preservative and as an antiseptic. You can find detailed UV spectra of Benzalkonium chloride and information about its various lambda maxima by visiting the following link.

Cetylpyridinium Chloride, also written as CPC, is a cationic quaternary ammonium compound with the chemical formula C₂₁H₃₈ClN. It is commonly used in mouthwashes, toothpastes, lozenges, throat sprays, breath sprays, and nasal sprays due to it’s antiseptic abilities. You can find detailed UV spectra of Cetylpyridinium Chloride and information about its various lambda maxima by visiting the following link.

Triton X100 is a nonionic surfactant with the chemical formula C₁₄H₂₂O(C₂H₄O)_n. It is primarily used as a detergent in laboratories, but it has a wide variety of laboratory uses. Commercially, it can be found in cleaning compounds. You can find detailed UV spectra of Triton X100 and information about its various lambda maxima by visiting the following link.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl^–. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

1-Pentanesulfonic acid is an organosulfonic acid with the chemical formula C₅H₁₂O₃S. It is primarily used in it’s sodium salt form as an anionic pairing agent. You can find detailed UV spectra of 1-Pentanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Hexanesulfonic acid is an alkyl sulfonate compound with the chemical formula C₆H₁₃O₃S^– (or C₆H₁₃NaO₃S as a sodium salt). It is primarily used in it’s sodium salt form as an ionic pairing agent in high-performance liquid chromatography.

1-Heptanesulfonic acid is an organosulfonic acid with the chemical formula C₇H₁₆O₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Heptanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Octanesulfonic acid is an anionic organosulfonic acid with the chemical formula C₈H₁₈O₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Heptanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Decanesulfonic acid is an alkyl sulfonic acid with the chemical formula C₁₀H₂₁NaO₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Decanesulfonic acid and information about its various lambda maxima by visiting the following link.

Sodium dodecyl sulfate (SDS), or sodium lauryl sulfate (SLS), is a surfactant used in cleaning, hygienic, and food products. It has a chemical formula C₁₂H₂₅NaSO₄, meaning it has twelve carbon hydrophobic tail attached to a polar sulfate. At low concentrations, it is used as a whipping aid and emulsifier in foods containing egg whites. It has a similar use in pharmaceutics as an ionic solubilizer and, also, an emulsifier. In laboratory uses, it is used as a component for lysing cells during RNA extraction or DNA extraction. You can find detailed UV spectra of Sodium dodecyl sulfate and information about its various lambda maxima by visiting the following link.

Benzalkonium chloride, Cetylpyridinium Chloride, Triton X100, 1-Pentanesulfonic acid, 1-Hexanesulfonic acid, sodium salt, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, Sodium dodecyl sulfate, 1-Octanesulfonic acid can be retained, separated, and analyzed using a reverse-phase Newcrom BH, 4.6 x 250 mm, 5 µm, 100 A, dual ended column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

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HPLC Method for Analysis of 1-Pentanesulfonic acid on Newcrom BH by SIELC Technologies

Pentanesulfonic acid, also known as Amyl sulfonic acid or Valeric acid, is an aliphatic sulfonic acid with the chemical formula C₅H₁₂O₃S. It has a five-carbon chain (which is where “penta-” comes from) and a sulfonic acid group attached (-SO3H), which is a strong acid group.

This compound is often used as an ion pairing reagent in reverse-phase chromatography, a type of high performance liquid chromatography (HPLC). As an ion-pairing reagent, it helps retain analytes with ionic (particularly cationic) properties on a reverse phase column by forming a neutral ion pair. This ion pair can then interact with the hydrophobic stationary phase and can be separated based on its hydrophobic properties.

It is also used in the synthesis of a variety of chemical compounds in the laboratory and industrial settings. As with all chemicals, it should be handled with appropriate care, considering its physical and chemical properties, and potential hazards.

You can find detailed UV spectra of 1-Pentanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Pentanesulfonic acid can be retained and analyzed using a mixed-mode Newcrom BH column. The mobile phase for this method consists of water, acetonitrile (MeCN), and Ammonium formate, which serves as a buffer. This analytical method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

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HPLC Method for Analysis of Sulfonic acids on BIST A+ Column by SIELC Technologies

Methanesulfonic acid, written on occasion as MsOH or MSA, is a non-volatile catalyst with the chemical formula CH₄O₃S. It is used primarily as a replacement for other acids in industrial and laboratory applications due to being biodegradable. It is used often in electroplating of tin and tin0lead soldiers sa well as a key ingredient in rust and scale removers.

1-Pentanesulfonic acid is an organosulfonic acid with the chemical formula C₅H₁₂O₃S. It is primarily used in it’s sodium salt form as an anionic pairing agent. You can find detailed UV spectra of 1-Pentanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Heptanesulfonic acid is an organosulfonic acid with the chemical formula C₇H₁₆O₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Heptanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Decanesulfonic acid is an alkyl sulfonic acid with the chemical formula C₁₀H₂₁NaO₃S. It is primarily used in it’s sodium salt form as an ion-pairing agent. You can find detailed UV spectra of 1-Decanesulfonic acid and information about its various lambda maxima by visiting the following link.

1-Dodecanesulfonic acid is an organosulfonic acid with the chemical formula C₁₂H₂₆O₃S. It is primarily used in it’s sodium salt form as an anionic surfactant and ion-pair reagent, especially in seapration of peptides and proteins in HPLC.

Using SIELC’s newly introduced BIST™ method, a mixture of these Sulfonic acids can be separated on a negatively-charged, cation-exchange BIST A+ column, contrary to conventional chromatographic wisdom. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include Calcium acetate and Magnesium acetate. Using this new and unique analysis method, these Sulfonic acids can be separated, retained, and detected through ELSD. This method is also compatible with Mass Spectrometry (LC-MS) and CAD.

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