Separation of Sulphonium Ions in Polar Organic Mode

The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both polar organic and ion-exchange modes in the separation of sulfonium ions. The high organic mobile phase retains methane sulfonic acid by a polar organic mechanism and separates methoxydimethylsulphonium and trimethylsulfphonium cations by polar organic and cation exchange modes. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN), and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD).


Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 35/65%
Buffer TFA
Flow Rate 1.5 ml/min
Detection ELSD


Class of Compounds Acid, Hydrophilic, Ionizable
Analyzing Compounds Sulphonium Ions, Methoxydimethylsulphonium, Trimethylsulfphonium
Application Analytes:
Methanesulfonic Acid
Sulfonium Ions