HPLC Method for Sodium, Phosphate, Chloride, Bromide, Nitrate, Sulfate, Iodine, Perchlorate, Iodide on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium, Phosphate, Chloride, Bromide, Nitrate, Sulfate, Iodine, Perchlorate, Iodide 

Sodium, which  has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

Phosphate, PO₄^3-, is a compound that plays an important part in biological energy transfer. It contains phosphorus, which is also a key component in bones and teeth. It is also a buffer, which helps maintain pH levels in a body.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl^–. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

Bromide is typically used to refer to a compound or molecule that contains a bromide anion: Br^–. It is often found in anticonvulsants, flame-retardant materials, and cell stains.

Nitrate, NO₃^–, is a compound of nitrogen and oxygen. It is essential as a nutrient in plants; therefore, it is often used in fertilizers. It is easily found in leafy greens. While it is important for cardiovascular health, too high of exposure to it can be dangerous.

Iodide, the Ionic form of Iodine, I^– is essential for thyroid hormone production. Lack of it can lead to iodine deficiency disorders. It is typically found in Iodized salt and occasionally as an antiseptic.

Sulfate is an ionic chemical with the formula SO₄^2-. In nature, it is usually found in geodes like gypsum. It is also created in atmosphere from sulfur dioxide. It is used for a variety of applications, but most notably, coloring wood and creating light-reflecting paints.

Perchlorate is an inorganic compound with the formula ClO₄^–. It is most typically used for explosives. For example, fireworks and rocket propellants. Due to it’s water solubility, it can easily contaminate it. When ingested, it can pose a health risk and cause developmental harm.

Sodium, Phosphate, Chloride, Bromide, Nitrate, Sulfate, Iodine, Perchlorate, Iodide can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Acetate buffer. Detection is performed using CAD.

HPLC Method for Iodine, Methimazole, Levothyroxine (T4), 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine dihydrate (DIT), 3-Iodo-L-tyrosine (MIT), Liothyronine (T3), Iodide on Primesep D by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Iodine, Methimazole, Levothyroxine (T4), 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine dihydrate (DIT), 3-Iodo-L-tyrosine (MIT), Liothyronine (T3), Iodide 

Iodine is an important element in production of thyroid hormones, T3 and T4, which are essential regulators of organism’s metabolism. The glands’ concentration of Iodide, hormones T3 and T4, along with their precursors: 3,5-Diiodo-L-thyronine (T2), 3,5-Diiodo-L-tyrosine (DIT), and 3-Iodo-L-tyrosine (MIT) can now be studied simultaneously with this HPLC method.  In order to analyze the hydrophobic hormones along with iodide (a very polar inorganic ion) a special mixed-mode column, Primesep D, was used.  This column provides both strong hydrophobic and anion exchange properties.  The Primesep D silica-bonded ligand is comprised of a long alkyl chain and an embedded amino functional group.  The TFA was used as an ionic modifier to provide stable acidic pH and sufficient ion strength for the mobile phase.  The mobile phase composition is suitable for UV, MS, ELSD, and CAD detection.

Halide ions are usually analyzed by ion-chromatography with a conductivity detector. Mixed-mode chromatography in combination with ELSD is offering a valuable alternative to ion-chromatography with conductivity detection. Chloride, bromide and iodide were separated on a Primesep SB mixed-mode anion-exchange column. Mobile phase is compatible with ELSD and LC/MS.