Phosphorous acid

phosphorous acid

CAS Number10294-56-1
Molecular FormulaH3O3P
Molecular Weight81.995
InChI KeyOJMIONKXNSYLSR-UHFFFAOYSA-N
LogP1.15
Synonyms
  • Phosphorous acid
  • 10294-56-1
  • Caswell No. 663I
  • EINECS 233-663-1
  • EPA Pesticide Chemical Code 076002
  • UNII-95E079716M
  • 858711-57-6

Applications:

HPLC Separation of Polar Pesticides on Newcrom B Column

November 19, 2020

 

 

 

 

 

 

 

Pesticide is a more generic term that includes herbicides, fungicides and insecticides in its definition. Herbicides are used to control unwanted plants, they are also known as weedkillers. Insecticides are used to kill insects. Fungicides are used to kill parasitic fungi. All are heavily used in agriculture. By using HPLC, many different pesticides can be separated and their retention characteristics controlled using the Newcrom B mixed-mode column.

Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm pH 3.0 Gradient  5 – 60 mM 15 min
Flow Rate 1 ml/min
Detection CAD

 

Condition 2

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer AmFm pH 3.0 Gradient  5 – 15 mM 10 min
Flow Rate 1 ml/min
Detection CAD

 

 

Description

Class of Compounds Pesticides, Herbicides, Fungicides, Insecticides
Analyzing Compounds Aminomethylphosphonic acid (AMPA), Bialaphos, Glyphosate, Phosphorous acid H3PO3, Fosetyl-Al, Bromide, Chlorate, Perchlorate, 3-Methylphosphinicopropionic acid (MPPA), Bromide

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
3-(Methylphosphinico)propionic acid
Aminomethylphosphonic acid (AMPA)
Bialaphos
Bromate
Bromide
Chlorate
Fosetyl-Al
Glyphosate
Perchlorate
Phosphorous acid
Sodium Bromate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Phosphoric and Phosphorous Acids on Newcrom B Column

March 11, 2020


HPLC.cloud
View on hplc.cloud

Condition

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer Formic Acid –  2%
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description

Class of Compounds Hydrophilic,  Acid
Analyzing Compounds Phosphoric acid,  Phosphorous acid

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Phosphoric Acid
Phosphorous acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Phosphorous and Phosphoric Acids

July 10, 2012

 

Application Notes: Phosphorus and phosphoric acid were separated on Obelisc N and Primesep D columns. On Obelisc N both acids retained by combination of HILIC and anion-exchange mechanisms. OnPrimesep D these inogranic acids are retained by anion-exchange mechanism. Both columns and methods can be used for analysis of hydrophilic organic and inorganic acids with multi-mode or single mode approach
Application Columns: Primesep D, Obelisc N
Application compounds: Phosphorous Acid, Phosphoric Acid
Detection technique: UV, LC/MS, ELSD/CAD

 

Condition 1

Column Primesep D, 3.2×50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm
Flow Rate 0.5 ml/min
Detection ELCD

 

Condition 2

Column Obelisc N, 4.6×50 mm, 5 µm, 100A
Mobile Phase  MeCN
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
 Acid, Fungicide,  Hydrophilic, Ionizable
Analyzing Compounds Phosphorous Acid,  Phosphoric Acids

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Phosphoric Acid
Phosphorous acid

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.