HPLC Separation of Phosphorous and Phosphoric Acids

  Application Notes: Phosphorus and phosphoric acid were separated on Obelisc N and Primesep D columns. On Obelisc N both acids retained by combination of HILIC and anion-exchange mechanisms. OnPrimesep D these inogranic acids are retained by anion-exchange mechanism. Both columns and methods can be used for analysis of hydrophilic organic and inorganic acids with multi-mode or single mode approach Application Columns: Primesep D, Obelisc N Application compounds: Phosphorous Acid, Phosphoric Acid Detection technique: UV, LC/MS, ELSD/CAD  

Condition 1

Column Primesep D, 3.2x50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm
Flow Rate 0.5 ml/min
Detection ELCD
 

Condition 2

Column Obelisc N, 4.6x50 mm, 5 µm, 100A
Mobile Phase  MeCN
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD
 

Description

Class of Compounds  Acid, Fungicide,  Hydrophilic, Ionizable
Analyzing Compounds Phosphorous Acid,  Phosphoric Acids
SIELC Technologies usually develops more than one method for each compound on our website. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound of interest.
Application Analytes:
Phosphoric Acid
Phosphorous acid

Application Detection:
UV Detection
ELSD/MS Detection