Application Notes: Phosphorus and phosphoric acid were separated on Obelisc N and Primesep D columns. On Obelisc N both acids retained by combination of HILIC and anion-exchange mechanisms. OnPrimesep D these inogranic acids are retained by anion-exchange mechanism. Both columns and methods can be used for analysis of hydrophilic organic and inorganic acids with multi-mode or single mode approach
Application Columns: Primesep D, Obelisc N
Application compounds: Phosphorous Acid, Phosphoric Acid
Detection technique: UV, LC/MS, ELSD/CAD
| Column | Primesep D, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | AmFm |
| Flow Rate | 0.5 ml/min |
| Detection | ELCD |
| Column | Obelisc N, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN |
| Buffer | AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Acid, Fungicide, Hydrophilic, Ionizable |
| Analyzing Compounds | Phosphorous Acid, Phosphoric Acids |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select options
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPhosphorous acid
UV Detection
