HPLC Separation of Phosphorous and Phosphoric Acids

  Application Notes: Phosphorus and phosphoric acid were separated on Obelisc N and Primesep D columns. On Obelisc N both acids retained by combination of HILIC and anion-exchange mechanisms. OnPrimesep D these inogranic acids are retained by anion-exchange mechanism. Both columns and methods can be used for analysis of hydrophilic organic and inorganic acids with multi-mode or single mode approach Application Columns: Primesep D, Obelisc N Application compounds: Phosphorous Acid, Phosphoric Acid Detection technique: UV, LC/MS, ELSD/CAD  

Condition 1

Column Primesep D, 3.2x50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmFm
Flow Rate 0.5 ml/min
Detection ELCD

Condition 2

Column Obelisc N, 4.6x50 mm, 5 µm, 100A
Mobile Phase  MeCN
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds  Acid, Fungicide,  Hydrophilic, Ionizable
Analyzing Compounds Phosphorous Acid,  Phosphoric Acids
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Application Analytes:
Phosphoric Acid
Phosphorous acid

Application Detection:
UV Detection
ELSD/MS Detection