HPLC Separation of Phosphoric and Phosphorous Acids on Newcrom B Column

HPLC Method for Phosphoric Acid, Phosphorous acid on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Phosphoric Acid, Phosphorous acid.

Phosphoric acid is an inorganic compound with chemical formula H3PO4. It is odorless and colorless, which leads to it’s common use in soft drunks to help preserve the product. It is also used in fertilizers, metal treatment, and corrosion inhibition. Excessive intake of it is not recommended.

Phosphorous acid is a compound with chemical formula H3PO3. It’s most important use is considered to be the production of basic lead phosphite, which is a stabilizer in PVC. It is also used as a reducing agent and in the production of synthetic fibers, organophosphorus pesticides, and the water treatment agent ATMP.

 

Condition
Column Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 20/80%
Buffer Formic Acid –  2%
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Description
Class of Compounds Hydrophilic,  Acid
Analyzing Compounds Phosphoric Acid, Phosphorous acid

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Phosphoric Acid
Phosphorous acid
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.