HPLC Determination of Chloride, Chlorate and Perchlorate on Newcrom B Column

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Main source of contamination of environment by perchlorates are rocket fuels, car airbags, and fireworks.
EPA developed regulation of the perchlorate level in drinking water which already adapted by several states.
The most convenient, universal and very sensitive way to measure perchlorate is chromatography.
SIELC developed a simple, rugged, and selective HPLC method which allows to measure perchlorate in different matrices including drinking water.
This method allows to measure simultaneously other chloro containing ions such as chloride, and chlorate.
The method shows high selectivity and specificity.
The mobile phase is a simple mixture of water acetonitrile and ammonium formate.
The column used in the analysis has advanced surface chemistry with long chain holding a terminal positively changed functional group.


Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer Gradient AmFm pH 3.0 –  40-100 mM , 10 min
Flow Rate 1.0 ml/min
Detection CAD


Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Sodium Chloride, Sodium Chlorate, Sodium Perchlorate

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Sodium Chlorate
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.