HPLC Separation of Chloride and Nitrate Ions

High nitrate concentrations in water and soil can be toxic to humans, fish and domestic animals.  Chloride can be used for analysis of water contamination. Primesep D column can be used to separate and quantify both ions.  The baseline separation between chloride and nitrate can be increased by decreasing the ammonium formate concentration in the mobile phase.  Evaporative Light Scattering Detection (ELSD) used.


Column Primesep D, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Hydrophilic, Ion
Analyzing Compounds Chloride, Nitrate
Application Analytes:
Nitric Acid